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Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF

Catalog #: 9224-FC Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9224-FC-050
Recombinant Cynomolgus Fc gamma RIII/CD16 Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma RIII (CD16) was immobilize on a His Tag antibody coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately
0.15-0.75 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIII (CD16) protein
Gly17-Gln208, with a C-terminal 6-His tag
Accession #
NP_001270121
N-terminal Sequence
Analysis
Gly17, Glu21 and Met18
Predicted Molecular Mass
23 kDa
SDS-PAGE
36-44 kDa, reducing conditions

Product Datasheets

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9224-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9224-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Lyophilized from a 0.2 μm filtered solution in PBS.

Scientific Data

Binding Activity Recombinant Cynomolgus Fc gamma RIII/CD16 Protein Binding Activity View Larger

When Recombinant Cynomolgus Fc gamma RIII (CD16) (Catalog # 9224-FC) was immobilized on a His Tag antibody (Catalog # MAB050) coated plate, it binds Biotinylated Human IgG with an ED50 of 0.15-0.75 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIII (CD16)

Fc gamma  RIII/CD16 is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). Mature cynomolgus Fc gamma  RIII consists of a 192 aa ECD with two C2-type
Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (4). In humans, Fc gamma  RIIIA/CD16a is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (5). Within the ECD, mature cynomolgus Fc gamma RIII shares 92% and 90% aa sequence identity with human Fc gamma  RIIIA and Fc gamma  RIIIB, respectively. Fc gamma RIII surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta (8, 9). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (10). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (11-14). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (14). Soluble Fc gamma  RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (12, 13). Cynomolgus Fc gamma RIII binds to cynomolgus IgG subclasses 1-4, to human IgG1 and 3, and more weakly to human IgG2 and 4 (15).

References
  1. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
  2. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
  3. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
  4. Rogers, K.A. et al. (2008) J. Immunol. 177:3848.
  5. Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
  6. Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
  7. Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664.
  8. Kim, M.-K. et al. (2003) Blood 101:4479.
  9. Lanier, L.L. et al. (1989) Nature 342:803.
  10. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.    
  11. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  12. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  13. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  14. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
  15. Warncke, M. et al. (2012) J. Immunol. 188:4405.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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