Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF

Name: Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF
Brand: R&D Systems
SKU: 10029-BC
Rating: 5 (1 reviews)

Catalog #: 10029-BC 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10029-BC-050

Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein Bioactivity

2 Images

All BCMA/TNFRSF17 Proteins and Enzymes

Product Details

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 30-180 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived BCMA/TNFRSF17 protein

Cynomolgus Monkey BCMA (Met1-Ile54) Accession # XP_005591343	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005591343

N-terminal Sequence
Analysis

Met1

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

33 kDa

SDS-PAGE

37-43 kDa, reducing conditions

Product Datasheets

10029-BC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10029-BC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Chimera (Catalog # 10029-BC) inhibits Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP) mediated proliferation of anti-IgM stimulated mouse B cells. The ED₅₀ for this effect is 30-180 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey BCMA/TNFRSF17 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-43 kDa and 75-85 kDa, respectively.

Reconstitution Calculator

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, also known as Tumor Necrosis Factor Receptor Superfamily member 17 (gene name TNSFR17), is a member of the TNFR superfamily, due to the presence of its TNFR motif (1). BCMA is a type III membrane protein containing one extracellular cysteine rich domain, a transmembrane domain, and an intracellular domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily (2, 3). This binding to APRIL and BAFF has been shown to stimulate IgM production in peripheral B cells and increase the survival of cultured B cells (3, 4). This data suggests that BCMA may play an important role in B cell development, function and regulation (5). BCMA expression has been found in immune organs and mature B cell lines (5). Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment (6). Within the ECD, cynomolgus monkey BCMA shares 89% sequence identity with human BCMA, 62% with mouse BCMA, and 59% with rat BCMA. The expression of BCMA has also been linked to various cancers, autoimmune disorders, and infectious diseases (7). Proteolytic shedding of the BCMA extracellular domain generated soluble BCMA (sBCMA) via direct cleavage by gamma -secretase, elevated sBCMA levels in serum may correlate with disease activity (8). More recently, BCMA has been indicated as a possible biomarker in various human immunological disease, and as a potential therapeutic target for multiple myeloma (MM) (9-11).

References

Hatzoglou, A. et al. (2000). J Immunol. 165:1322.
Schiemann, B. et al. (2001). Science. 293:2111.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Huang, H. et al. (2013). PNAS. 110:10928.
Laâbi, Y. et al. (1994). Nucleic Acids Res. 22:1147.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Coquery, C.M. et al. (2012) Crit Rev Immunol. 32:287.
Laurent, S.A. et al. (2015) Nat Commun. 6:7333.
Thaler, F.S. et al. (2017) Neuro Oncol. 19:1618.
Seckinger, A. et al. (2017) Cancer Cell. 31:396.
Lee, L. et al. (2018) Blood. 131:746.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17