Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF

Catalog #: 10225-IL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10225-IL-050

Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 5-40 ng/mL

Source

Chinese Hamster Ovary cell line, CHO-derived IL-17F protein
Arg31-Gln163, with a C-terminal 6-His tag

Accession #

NP_001248216.1

N-terminal Sequence
Analysis

Arg31

Predicted Molecular Mass

16 kDa

SDS-PAGE

17-25 kDa, under reducing conditions

Product Datasheets

10225-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10225-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Cynomolgus Monkey/Rhesus Macaque IL-17F (Catalog # 10225-IL) induces IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 5-40 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus/Rhesus Macaque IL-17F His-tag (Catalog # 10225-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa and 30-50 kDa, respectively.

Reconstitution Calculator

Background: IL-17F

The Interleukin 17 (IL-17) protein family, composed of six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL-17B which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Cynomolgus IL-17F cDNA encodes a 163 amino acid (aa) protein with a putative 30 aa signal peptide. Mature cynomolgus IL-17F shares 96% and 100% aa sequence identity with human and rhesus macaque IL-17F, respectively. IL-17F is expressed in activated CD4⁺ T-cells and activated monocytes. Five receptors (IL-17 RA-RE) have been identified. Although the ligands for IL-17 RD and RE are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL‑17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL‑17F (3, 4). The biological activities mediated by IL-17F are similar to those of IL-17A. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (5). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (6).

References