Recombinant Cynomolgus/Rhesus Macaque TrkA Fc Protein, CF

Catalog # Availability Size / Price Qty
11316-TK-050
Recombinant Cynomolgus Monkey/Rhesus Macaque TrkA Fc Chimera Protein Bioactivity.
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Recombinant Cynomolgus/Rhesus Macaque TrkA Fc Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 8.00-80.0 ng/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF Protein (Catalog # 256-GF).
Source
Chinese Hamster Ovary cell line, CHO-derived TrkA protein
Cynomolgus Monkey/Rhesus Macaque TrkA
(Ser34-Gly423)
Accession # XP_005541454.3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser34
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
69 kDa
SDS-PAGE
93-103 kDa, under reducing conditions.

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11316-TK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11316-TK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 8.00-80.0 ng/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF Protein (256-GF).

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus/Rhesus Macaque TrkA Fc Chimera Protein (Catalog # 11316-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-103 kDa and 180-200 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: TrkA

TrkA (Tyrosine kinase receptor A), also known as High affinity NGF receptor, is a member of the neurotrophic tyrosine kinase receptor family that has three members,  TrkA, Trk B and Trk C, which preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively (1). All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (1). Mature cynomolgus TrkA consists of a 391 amino acid (aa) extracellular domain (ECD) which shares 98.5% aa identity with human TrkA.  NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily (2). NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when being expressed independent of Trk (3).

References
  1. Shelton D.L. et al. (1995) J. Neurosci. 15:477.
  2. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  3. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

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