Recombinant Feline SCF Protein Summary
Product Specifications
Lys26-Ala190, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2268-SC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2268-SC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SCF/c-kit Ligand
Feline SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1 - 4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are two intrachain disulfide bonds and four alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28 - 40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of feline SCF has been identified (5). It too, is membrane bound and contains 246 aa residues. It will not give rise to a soluble form, since alternate splicing removes the proteolytic cleavage site used in the long form. The ratio of long form to short form varies tissue to tissue (1). Soluble feline SCF shares 93%, 93%, 90%, 87%, and 78% aa sequence identity with porcine, canine, bovine, human and mouse SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).
- Broudy, V.C. (1997) Blood 90:1345.
- Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
- Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
- Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
- Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
- Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
- Kapur, R. et al. (1998) Blood 91:879.
Citations for Recombinant Feline SCF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Intratumoral heterogeneity of c-KIT mutations in a feline splenic mast cell tumor and their functional effects on cell proliferation
Authors: Y Hasegawa, K Shosu, K Tsuji, Y Shimoyama, TS Miyama, K Baba, M Okuda, K Itamoto, M Igase, T Mizuno
Scientific Reports, 2022-09-22;12(1):15791.
Species: Mouse
Sample Types: Whole Cells
Applications: Proliferation -
Mutations in the fifth immunoglobulin-like domain of kit are common and potentially sensitive to imatinib mesylate in feline mast cell tumours.
Authors: Isotani M, Yamada O, Lachowicz JL, Tamura K, Yagihara H, Fujino Y, Ono K, Washizu T, Bonkobara M
Br. J. Haematol., 2009-10-05;148(1):144-53.
Species: Feline
Sample Types: Whole Cells
Applications: Bioassay
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