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Recombinant Human ACE-2 His-tag Biotinylated Protein, CF

Catalog #: BT933
1 Citation Datasheet / COA / SDS
Biotinylated via Amines
Catalog # Availability Size / Price Qty
BT933-020
Recombinant Human ACE-2 His-tag Biotinylated Protein Binding Activity
3 Images
Product Details
Citations (1)
FAQs
Supplemental Products
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ACE-2 His-tag Biotinylated Protein, CF Summary

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Biotinylated Recombinant Human ACE-2 His-tag (Catalog # BT933) binds Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera  (Catalog # 10499-CV) in a functional ELISA. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >600 pmol/min/μg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human ACE-2 protein
Gln18-Ser740
with a C-terminal 10-His tag
Accession #
Q9BYF1.2
N-terminal Sequence
Analysis
No results obtained: Gln18 predicted.
Structure / Form
Biotinylated via amines.
Predicted Molecular Mass
85 kDa
SDS-PAGE
100-110 kDa, under reducing conditions

Product Datasheets

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BT933

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT933

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity Recombinant Human ACE-2 His-tag Biotinylated Protein Binding Activity View Larger

Biotinylated Recombinant Human ACE-2 His-tag (Catalog # BT933) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) in a functional ELISA.

Enzyme Activity Recombinant Human ACE-2 His-tag Biotinylated Protein Enzyme Activity View Larger

Biotinylated Recombinant Human ACE‑2 His-tag (Catalog # BT933) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).

SDS-PAGE Recombinant Human ACE-2 His-tag Biotinylated Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human ACE-2 His-tag Biotinylated Protein (Catalog # BT933) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~ 104 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12).  Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References
  1. Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
  2. Yan, R. et al. (2020) Science 367:1444.
  3. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Hoffman, M. et al. (2020) Cell.181:1.
  6. Wrapp, D. et al. (2020) Science 367:1260.
  7. Imai, Y. et al. (2005) Nature 436:112.
  8. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  9. Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
  10. Jia, H. et al. (2016) Shock. 46:239.
  11. Cole-Jeffrey, C.T. et al. (2015) J. Cardiovasc. Pharmacol. 66:540.
  12. Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
  13. Crackower, M.A. et al. (2002) Nature 417:822.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citation for Recombinant Human ACE-2 His-tag Biotinylated Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human antibody recognition and neutralization mode on the NTD and RBD domains of SARS-CoV-2 spike protein
    Authors: R Otsubo, T Minamitani, K Kobiyama, J Fujita, T Ito, S Ueno, I Anzai, H Tanino, H Aoyama, Y Matsuura, K Namba, KI Imadome, KJ Ishii, K Tsumoto, W Kamitani, T Yasui
    Scientific Reports, 2022-11-22;12(1):20120.
    Species: Human
    Sample Types:
    Applications: Binding Assay

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