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Recombinant Human Adiponectin/Acrp30 Protein, CF

Catalog #: 1065-AP
19 Citations 2 Reviews Datasheet / COA / SDS
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1065-AP-050
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Citations (19)
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Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Adiponectin/Acrp30 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit glucose production by rat hepatocytes. Zhou, H. et al. (2005) Biochem. Biophys. Res. Commun. 338:793. The ED50 for this effect is 4‑20 µg/mL. Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046. The ED50 for this effect is 1-5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Adiponectin/Acrp30 protein
Glu19-Asn244, with a C-terminal 6-His tag
Accession #
Q15848
N-terminal Sequence
Analysis
Glu19
Structure / Form
Oligomer
Predicted Molecular Mass
25.4 kDa
SDS-PAGE
27-35 kDa, reducing conditions

Product Datasheets

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1065-AP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1065-AP

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N‑terminal collagenous region and a 137 aa C‑terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Citations for Recombinant Human Adiponectin/Acrp30 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion
    Authors: M Soezi, S Piri-Gavga, M Ghanei, MD Omrani, B Soltanmoha, KP Bagheri, RA Cohan, F Vaziri, SD Siadat, A Fateh, S Khatami, M Azizi, F Rahimi-Jam
    BMC biotechnology, 2022-10-28;22(1):31.
    Species: N/A
    Sample Types: Antibody
    Applications: ELISA Capture Control
  2. Distribution of Adiponectin Receptors 1 and 2 in the Rat Olfactory Bulb and the Effect of Adiponectin Injection on Insulin Receptor Expression
    Authors: A Miranda-Ma, OF Mercado-Gó, V Arriaga-Áv, R Guevara-Gu
    Int J Endocrinol, 2017-12-31;2017(0):4892609.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Endocrine disrupting compounds modulates adiponectin secretion, expression of its receptors and action on steroidogenesis in ovarian follicle
    Authors: A Rak, K Zajda, E? Gregoraszc
    Reprod. Toxicol, 2017-03-08;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Decreased Adiponectin-Mediated Signaling Through the AdipoR2 Pathway Is Associated With Carotid Plaque Instability
    Authors: K Gasbarrino, H Zheng, A Hafiane, JP Veinot, C Lai, SS Daskalopou
    Stroke, 2017-03-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity
    Authors: S Fu, H Xu, M Gu, C Liu, Q Wang, X Wan, Y Chen, Q Chen, Y Peng, Z Cai, J Zhou, Z Wang
    Sci Rep, 2017-03-03;7(0):43771.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the obesity paradox
    Authors: R Ito, S Narita, M Huang, T Nara, K Numakura, K Takayama, H Tsuruta, A Maeno, M Saito, T Inoue, N Tsuchiya, S Satoh, T Habuchi
    PLoS ONE, 2017-02-08;12(2):e0171615.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Beneficial effects of adiponectin on periodontal ligament cells under normal and regenerative conditions.
    Authors: Nokhbehsaim M, Keser S, Nogueira A, Cirelli J, Jepsen S, Jager A, Eick S, Deschner J
    J Diabetes Res, 2014-07-13;2014(0):796565.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Adiponectin enhances intercellular adhesion molecule-1 expression and promotes monocyte adhesion in human synovial fibroblasts.
    Authors: Chen H, Tsou H, Chen J, Shih J, Chen Y, Tang C
    PLoS ONE, 2014-03-25;9(3):e92741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Functional effects of adiponectin on endothelial progenitor cells.
    Authors: Lavoie V, Kernaleguen AE, Charron G, Farhat N, Cossette M, Mamarbachi AM, Allen BG, Rheaume E, Tardif JC
    Obesity (Silver Spring), 2010-09-02;19(4):722-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  10. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Applications: ELISA (Standard)
  11. Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes.
    Authors: von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM
    Diabetes, 2009-06-09;58(9):2093-9.
    Applications: Western Blot
  12. Adiponectin upregulates monocytic activin A but systemic levels are not altered in obesity or type 2 diabetes.
    Authors: Weigert J, Neumeier M, Wanninger J, Schober F, Sporrer D, Weber M, Schramm A, Wurm S, Stogbauer F, Filarsky M, Schaffler A, Aslanidis C, Scholmerich J, Buechler C
    Cytokine, 2009-01-06;45(2):86-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Adiponectin plays a protective role in caerulein-induced acute pancreatitis in mice fed a high-fat diet.
    Authors: Araki H, Nishihara T, Matsuda M, Fukuhara A, Kihara S, Funahashi T, Kataoka TR, Kamada Y, Kiyohara T, Tamura S, Hayashi N, Shimomura I
    Gut, 2008-06-25;57(10):1431-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Adiponectin reduces plasma triglyceride by increasing VLDL triglyceride catabolism.
    Authors: Qiao L, Zou C, van der Westhuyzen DR, Shao J
    Diabetes, 2008-03-28;57(7):1824-33.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Adiponectin levels measured in dried blood spot samples from neonates born small and appropriate for gestational age.
    Authors: Klamer A, Skogstrand K, Hougaard DM, Norgaard-Petersen B, Juul A, Greisen G
    Eur. J. Endocrinol., 2007-08-01;157(2):189-94.
    Applications: ELISA (Standard)
  16. Apolipoprotein A-I stimulates AMP-activated protein kinase and improves glucose metabolism.
    Authors: Han R, Lai R, Ding Q, Wang Z, Luo X, Zhang Y, Cui G, He J, Liu W, Chen Y
    Diabetologia, 2007-07-18;50(9):1960-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The potential of adiponectin in driving arthritis.
    Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
    J. Immunol., 2006-04-01;176(7):4468-78.
    Species: Human
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, Western Blot
  18. Chemokine induction by the adipocyte-derived cytokine adiponectin.
    Authors: Rovin BH, Song H
    Clin. Immunol., 2006-02-28;120(1):99-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
    Authors: Iversen PO, Wiig H
    Clin. Cancer Res., 2005-10-01;11(19):6793-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human Adiponectin/Acrp30 Protein, CF

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Recombinant Human Adiponectin/Acrp30 Protein, CF
By Anonymous on 09/04/2019
Application: Immunoassay Standard
Immunoassay Standard Recombinant Human Adiponectin/Acrp30 Protein, CF 1065-AP
Recombinant Human Adiponectin/Acrp30 Protein, CF
By Balaji Mahender on 12/20/2017
Application: Immunoassay Standard