Recombinant Human AMIGO3 Fc Chimera Protein, CF

Recombinant Human AMIGO3 Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: AMIGO3

AMIGO3 (Amphoterin-induced protein 3), also called Alivin-3, is a 68 kDa single-pass type I membrane cell adhesion protein. AMIGO3 belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Human AMIGO3 cDNA encodes 504 amino acids (aa) including a 19 aa signal sequence, 264 aa extracelluar domain, 21 aa transmembrane domain and 100 aa cytoplasmic domain. Human AMIGO3 shares 77.2% and 79.1% aa sequence identity with mouse and rat AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 is expressed in brain tissues, also found in liver, kidney and spleen (3). AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (4). Our in-house data showed that AMIGO3 inhibited anti-CD3 induced IL-2 secretion on CD3⁺ cells, suggesting that AMIGO3 may be involved in T cell activation.