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Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

Catalog #: 2334-ZN
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2334-ZN-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >125 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Aminopeptidase PILS/ARTS1 protein
Ala37-Met941, with a C-terminal 10-His tag
Accession #
EAW96077
N-terminal Sequence
Analysis
Ala37
Predicted Molecular Mass
105 kDa
SDS-PAGE
108 kDa, reducing conditions

Product Datasheets

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2334-ZN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2334-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Human Aminopeptidase PILS/ARTS1 (rhARTS1) (Catalog # 2334-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhARTS1 to 2 µg/mL in Assay Buffer.
  2. Dilute Substrate to 600 µM in Assay Buffer.
  3. Load 50 µL of 2 µg/mL rhARTS1 into the plate, and start the reaction by adding 50 µL of 600 µM Substrate. Include a Substrate Blank containing Assay Buffer and Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (respectively), in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhARTS1: 0.1 µg
  • Substrate: 300 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aminopeptidase PILS/ARTS1

The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2-4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2-5).

References
  1. Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
  2. Cui, X. et al. (2002) J. Clin. Invest. 110:515.
  3. York, I.A. et al. (2002) Nat. Immunol. 3:1177.
  4. Serwold, T. et al. (2002) Nature 419:480.
  5. Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Entrez Gene IDs
51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)
Alternate Names
Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Citations for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Modulators of hERAP2 discovered by high-throughput screening
    Authors: M Laura, G Ronan, LB Vy, G Valentin, CA Omar, C Virgyl, C Piveteau, R Melissa, F Charlotte, W Sandrine, C Julie, DR Julie, B Damien, L Florence, VE Peter, D Benoit, DP Rebecca
    European Journal of Medicinal Chemistry, 2020-12-11;211(0):113053.
    Applications: Bioassay
  2. ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope
    Authors: K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert
    Eur. J. Immunol., 2019-11-27;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  3. Sequence-specific alterations of epitope production by HIV protease inhibitors.
    Authors: Kourjian, Georgio, Xu, Yang, Mondesire-Crump, Ijah, Shimada, Mariko, Gourdain, Pauline, Le Gall, Sylvie
    J Immunol, 2014-03-10;192(8):3496-506.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  4. CHR-2797: an antiproliferative aminopeptidase inhibitor that leads to amino acid deprivation in human leukemic cells.
    Authors: Krige D, Needham LA, Bawden LJ, Flores N, Farmer H, Miles LE, Stone E, Callaghan J, Chandler S, Clark VL, Kirwin-Jones P, Legris V, Owen J, Patel T, Wood S, Box G, Laber D, Odedra R, Wright A, Wood LM, Eccles SA, Bone EA, Ayscough A, Drummond AH
    Cancer Res., 2008-08-15;68(16):6669-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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