Recombinant Human Annexin A10 Protein, CF

Catalog #: 9416-AN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9416-AN-050

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human Annexin A10 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived human Annexin A10 protein
Met1-Tyr324, with a C-terminal 6-His tag

Accession #

Q9UJ72

N-terminal Sequence
Analysis

Met1

Predicted Molecular Mass

38 kDa

SDS-PAGE

34 kDa, reducing conditions

Product Datasheets

9416-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9416-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Annexin A10

Human Annexin A10 (ANXA10), also known as Annexin 10 or Annexin-14, is a 37 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins preferentially being located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (2). Human Annexin A10 shares approximately 89% and 88% aa sequence identity with mouse and rat Annexin A10 respectively.

References