Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein Summary
Product Specifications
| HA (YPYDVPDYA) |
GCN4-IZ | GGGSGGGSGGGS | Human April (Ala105-Leu250) Accession # O75888 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5860-AP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
5860-AP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: APRIL/TNFSF13
APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily (1). It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule (2-4). Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL (2, 3). Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R (2, 5, 6). APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA (6, 7). The interaction with HSPGs induces APRIL oligomerization, and this augments TACI-, or BMCA-mediated effects (7, 8). HSPGs are also critical for the tumor growth-promoting effects attributed to APRIL (6). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (10). TWE-PRIL is a bioactive hybrid protein produced by gene splicing. It consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (11). TWE-PRIL is expressed in monocytes and activated T cells and, in contrast to APRIL, is presented on the cell surface (11). APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses (2, 12, 13). In the context of autoimmune disorders, however, APRIL can inhibit pathologic humoral responses as well as disease progression (14). Its expression by CD4+ T cells inhibits the production of Th2 cytokines and allergic inflammation (15). APRIL levels are elevated in the serum during coronary artery disease (16), and it is also elevated in many cancers primarily due to expression by tumor-infiltrating neutrophils (4, 7, 17).
- Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
- Yu, G. et al. (2000) Nat. Immunol. 1:252.
- Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
- Kelly, K. et al. (2000) Cancer Res. 60:1021.
- Day, E.S. et al. (2005) Biochemistry 44:1919.
- Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
- Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
- Schwaller, J. et al. (2007) Blood 109:331.
- Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
- Benson, M.J. et al. (2008) J. Immunol. 180:3655.
- He, B. et al. (2004) J. Immunol. 172:3268.
- Fernandez, L. et al. (2012) Ann. Rheum. Dis. 72:1367.
- Xiao, Y. et al. (2011) Eur. J. Immunol. 41:164.
- Sandberg, W.J. et al. (2009) Thromb. Haemost. 102:704.
- Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
Citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Macrophages drive KSHV B cell latency
Authors: Szymula, A;Samayoa-Reyes, G;Ogolla, S;Liu, B;Li, S;George, A;Van Sciver, N;Rochford, R;Simas, JP;Kaye, KM;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A BAFF/APRIL axis regulates obesogenic diet-driven weight gain
Authors: CC Chan, ITW Harley, PT Pfluger, A Trompette, TE Stankiewic, JL Allen, ME Moreno-Fer, MSMA Damen, JR Oates, PC Alarcon, JR Doll, MJ Flick, LM Flick, J Sanchez-Gu, R Mukherjee, R Karns, M Helmrath, TH Inge, SP Weisberg, SJ Pamp, DA Relman, RJ Seeley, MH Tschöp, CL Karp, S Divanovic
Nature Communications, 2021-05-18;12(1):2911.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
APRIL and BAFF: novel biomarkers for central nervous system lymphoma
Authors: M Mulazzani, M Huber, S Borchard, S Langer, B Angele, E Schuh, E Meinl, M Dreyling, T Birnbaum, A Straube, U Koedel, L von Baumga
J Hematol Oncol, 2019-10-15;12(1):102.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica
Authors: R Wilson, M Makuch, AK Kienzler, J Varley, J Taylor, M Woodhall, J Palace, MI Leite, P Waters, SR Irani
Brain, 2018-04-01;141(4):1063-1074.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL
Authors: D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius
Mol. Cancer Ther., 2017-08-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
Leukemia, 2015-07-03;30(1):163-72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TACI expression and signaling in chronic lymphocytic leukemia.
Authors: Mamara, Antigoni, Germenis, Anastasi, Kompoti, Maria, Palassopoulou, Maria, Mandala, Eudokia, Banti, Anastasi, Giannakoulas, Nikolaos, Speletas, Matthaio
J Immunol Res, 2015-04-09;2015(0):478753.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles.
Authors: Deknuydt F, Nordstrom T, Riesbeck K
J Leukoc Biol, 2014-02-18;95(6):983-91.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL.
Authors: Osorio C, Chacon P, White M, Kisiswa L, Wyatt S, Rodriguez-Tebar A, Davies A
Mol Cell Neurosci, 2014-01-18;59(0):24-36.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications.
Authors: Tai Y, Landesman Y, Acharya C, Calle Y, Zhong M, Cea M, Tannenbaum D, Cagnetta A, Reagan M, Munshi A, Senapedis W, Saint-Martin J, Kashyap T, Shacham S, Kauffman M, Gu Y, Wu L, Ghobrial I, Zhan F, Kung A, Schey S, Richardson P, Munshi N, Anderson K
Leukemia, 2013-04-16;28(1):155-65.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the meaning of "HA (YPYDVPDYA)" in the Source section of the datasheet?
HA (YPYDVPDYA) stands for Hemagglutinin, which consists of amino acids Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. It is a popular epitope tag in proteins with mammalian expression systems.
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What is the structure of the Recombinant Human APRIL/TNFSF13 Protein (Catalog # 5860-AP)?
We expect this Catalog # 5860-AP to be a non-covalently associated homotrimer as provided.
Reviews for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
Average Rating: 5 (Based on 12 Reviews)
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Reason for Rating: Reliably promotes neurite outgrown of newborn mouse sympathetic neurons of the superior cervical ganglion.
Soluble APRIL enhances NGF-promoted axon growth from SCG neurons. (A) Images of representative calcein-labelled newborn SCG neurons incubated for 16 h in medium containing 0.1 ng/ml NGF and 25 µM Boc-D-FMK with and without 100 ng/ml soluble recombinant APRIL. Scale bar:100 µm. (B) Bar charts of total length of axons in the arbors of P0 SCG neurons grown for 16 h in medium containing 0.1 ng/ml NGF, 25 µM Boc-D-FMK and different concentrations of APRIL.
