Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Name: Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 193-BC
Rating: 5 (2 reviews)

Catalog #: 193-BC
4 Citations 2 Reviews Datasheet / COA / SDS

Analyzed by SEC-MALS

Catalog #	Availability	Size / Price	Qty
193-BC-050

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Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein SEC-MALS.

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All BCMA/TNFRSF17 Proteins and Enzymes

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF Summary

Why choose R&D Systems BCMA Protein?

Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

NEW! Fluorescent labeled BCMA designed for validating expression of a BCMA chimeric antigen receptor (CAR). Fluorescent BCMA Proteins

Avi-tag BCMA protein is also available with the same activity as the unlabeled BCMA protein and consistent biotinylation Avi-tag BCMA

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.

Source

Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein

Human BCMA (Met1-Ala54) Accession # Q6PE46	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6PE46

N-terminal Sequence
Analysis

Met1

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-45 kDa, reducing conditions

Product Datasheets

193-BC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

193-BC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS

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Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) has a molecular weight (MW) of 70.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity

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Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED₅₀ for this effect is 0.01-0.4 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.

Reconstitution Calculator

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

Related Research Areas

Citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Generation and validation of structurally defined antibody-siRNA conjugates
Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader
Nucleic Acids Res., 2020-06-04;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture
Suppressed humoral immunity is associated with dengue nonstructural protein NS1-elicited anti-death receptor antibody fractions in mice
Authors: CL Tsai, DS Sun, MT Su, TS Lien, YH Chen, CY Lin, CH Huang, CC King, CR Li, TH Chen, YH Chiu, CC Lu, HH Chang
Sci Rep, 2020-04-14;10(1):6294.
Species: Human, Rabbit
Sample Types: Protein, Serum
Applications: ELISA Capture
Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma
Authors: DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone
Oncotarget, 2018-05-25;9(40):25764-25780.
Species: Human
Sample Types: Protein
Applications: CAR-T (SPR)
Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL.
Authors: Isik G, Sliepen K, van Montfort T, Sanders R
PLoS ONE, 2014-09-23;9(9):e107683.
Species: Human
Sample Types: Protein
Applications: Bioassay