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Recombinant Human BCMA/TNFRSF17 Protein, Atto 488 Conjugate

Catalog #: ATJ193 Datasheet / COA / SDS
Fc Chimera
Catalog # Availability Size / Price Qty
ATJ193-020
Detection of Human BCMA Antibody with Recombinant BCMA/TNFRSF17 Fc Chimera Atto 488 Protein by Flow Cytometry
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Recombinant Human BCMA/TNFRSF17 Protein, Atto 488 Conjugate Summary

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by flow cytometry for its ability to bind anti-Human BCMA Monoclonal Antibody conjugated fluorescent beads.
Source
Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein
Human BCMA/TNFRSF17
(Met1-Ala54)
Accession # Q6PE46		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
Q6PE46
N-terminal Sequence
Analysis
Met1
Structure / Form
Disulfide-linked homodimer, labeled with Atto 488 via amines

Excitation Wavelength: 501 nm
Emission Wavelength: 523 nm
Predicted Molecular Mass
32 kDa (monomer)
SDS-PAGE
35-45 kDa, under reducing conditions

Product Datasheets

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ATJ193

Product Datasheet
COA
SDS

ATJ193

Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Flow Cytometry Detection of Human BCMA Antibody with Recombinant BCMA/TNFRSF17 Fc Chimera Atto 488 Protein by Flow Cytometry View Larger

Fluorescent beads conjugated to anti-Human BCMA Monoclonal Antibody were stained with (A) Recombinant Human BCMA/TNFRSF17 Fc Chimera Atto 488 Protein (Catalog # ATJ193) or (B) unstained.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human BCMA/TNFRSF17 Fc Chimera Atto 488 (ATJ193) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, also known as Tumor Necrosis Factor Receptor Superfamily member 17 (gene name TNSFR17), is a member of the TNFR superfamily, due to the presence of its TNFR motif (1). BCMA is a type III membrane protein containing one extracellular cysteine rich domain, a transmembrane domain, and an intracellular domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily (2, 3). This binding to APRIL and BAFF has been shown to stimulate IgM production in peripheral B cells and increase the survival of cultured B cells (3, 4). This data suggests that BCMA may play an important role in B cell development, function, and regulation (5). BCMA expression has been found in immune organs and mature B cell lines (5). Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment (6). Within the ECD, human and mouse BCMA shares 62% amino acid identity. The expression of BCMA has also been linked to various cancers, autoimmune disorders, and infectious diseases (7). Proteolytic shedding of the BCMA extracellular domain generats soluble BCMA (sBCMA) via direct cleavage by gamma -secretase, and elevated sBCMA levels in serum may correlate with disease activity (8). More recently, BCMA has been indicated as a possible biomarker in various human immunological disease, and as a potential therapeutic target for multiple myeloma (MM) (9-11).

References
  1. Hatzoglou, A. et al. (2000). J Immunol. 165:1322.
  2. Schiemann, B. et al. (2001). Science. 293:2111.
  3. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  4. Huang, H. et al. (2013). PNAS. 110:10928.
  5. Laâbi, Y. et al. (1994). Nucleic Acids Res. 22:1147.
  6. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  7. Coquery, C.M. et al. (2012) Crit Rev Immunol. 32:287.
  8. Laurent, S.A. et al. (2015) Nat Commun. 6:7333.
  9. Thaler, F.S. et al. (2017) Neuro Oncol. 19:1618.
  10. Seckinger, A. et al. (2017) Cancer Cell. 31:396.
  11. Lee, L. et al. (2018) Blood. 131:746.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

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