Recombinant Human BMP-4 Animal-Free Protein

Name: Recombinant Human BMP-4 Animal-Free Protein
Brand: R&D Systems
SKU: AFL314E
Price: 283 USD

Catalog #: AFL314E
1 Citation Datasheet / COA / SDS

GMP Version Available: 314E-GMP

GMP

Intended for preclinical researchers who may transition to GMP BMP-4 for their clinical work

Catalog #	Availability	Size / Price	Qty
AFL314E-010
AFL314E-050
AFL314E-01M
AFL314E-250

Request an Animal-Free Protein Sample. For a limited time, we are offering customers the opportunity to try a sample of any Animal-Free RUO protein at no charge. Request a sample of Animal-Free BMP-4.

Recombinant Human BMP-4 Animal-Free Protein Bioactivity

3 Images

All BMP-4 Proteins and Enzymes

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-4 Animal-Free Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 3-21 ng/mL.

Source

E. coli-derived human BMP-4 protein
Lys303-Arg408
Produced using non-animal reagents in an animal-free laboratory.

Accession #

P12644.1

N-terminal Sequence
Analysis

Lys303

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

12 kDa

SDS-PAGE

8-9 kDa, under reducing conditions

Product Datasheets

AFL314E

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL314E

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100-200 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 3-21 ng/mL.

SDS-PAGE

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2 μg/lane of Animal-Free^TM Recombinant Human BMP-4 Protein (AFL314E) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively.

Bioactivity

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Equivalent bioactivity of GMP (314-GMP), Animal-Free (Catalog # AFL314E) and RUO (314-BPE) grades of Recombinant Human BMP-4 as measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells (orange, green, red, respectively).

Reconstitution Calculator

Background: BMP-4

Bone morphogenetic protein 4 (BMP-4) is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). It is an integral part of many stem cell differentiation pathways, including lung tissue, (6), adipogenesis (7) and osteogenesis (8, 9). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 amino acid (aa) mature protein (10). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (11-13). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. In Xenopus, BMP-4 dimers provide ventralizing signals for existing mesoderm (14). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (15, 16). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (17-19).

References

Zhang, P. et al. (2008) Blood 111:1933.
Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
Sadlon, T.J. et al. (2004) Stem Cells 22:457.
Frank, D.B. et al. (2005) Circ. Res. 97:496.
Lee, J-H. et al. (2014) Cell. 156:440.
Tang, QQ. et Lane MD. (2012) Annu Rev. Biochem. 81:715.
Urist, MR. (1965). Science. 150:893.
Bandyopadhyay, A. et al. (2006) Plos Genetics. 2:e216.
Wozney, J. et al. (1988) Science 242:1528.
Cui, Y. et al. (1998) EMBO J. 17:4735.
Cui, Y. et al. (2001) Genes Dev. 15:2797.
Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
Chen, D. et al. (2004) Growth Factors 22:233.
Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

Long Name

Bone Morphogenetic Protein 4

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Alternate Names

BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Related Research Areas

Citation for Recombinant Human BMP-4 Animal-Free Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

TLR2 regulates hair follicle cycle and regeneration via BMP signaling
Authors: Xiong, L;Zhevlakova, I;West, XZ;Gao, D;Murtazina, R;Horak, A;Brown, JM;Molokotina, I;Podrez, EA;Byzova, TV;
eLife
Species: Human hepegivirus
Sample Types: Whole Cells
Applications: Bioassay