Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF
Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF Summary
Learn more about Avi-tag Biotinylated ProteinsProduct Specifications
| Human BTN2A1 (Gln29-Ala248) Accession # Q7KYR7.3 | IEGRMD | Human IgG1 (Pro100-Lys330) | Avi-tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI10909
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag Protein (Catalog # AVI10909) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa to 130-160 kDa, respectively.
Reconstitution Calculator
Background: BTN2A1
Butyrophilin 2A1 (BTN2A1) is a type I transmembrane glycoprotein of the butyrophilin family within the Ig-superfamily. There are over 13 Butyrophilin and the closely related butyrophilin-like (BTNL) molecules and they have been identified as immune checkpoint receptors involved in modulating T cell function (1,2). Mature human BTN2A1 consists of an extracellular domain (ECD) with two immunoglobulin-like domains (one IgV and one IgC), a transmembrane segment, and a cytoplasmic region with a B30.2 domain (3). Alternative splicing generates additional isoforms of BTN2A1 that lack the first Ig-like domain or the transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (4, 5). BTN2A1 binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (4). A polymorphism of BTN2A1 has been associated with metabolic syndromes, type II diabetes mellitus, chronic kidney disease, and hypertension (6, 7). Our Avi-tag Biotinylated BTN2A1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
- Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
- Afrache, H. et al. (2012) Immunogenetics 64:781.
- Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
- Malcherek, G. et al. (2007) J. Immunol. 179:3804.
- Cavaletto, M. et al. (2002) Proteomics 2:850.
- Oguri, M. et al.(2011) J. Med. Genet. 48:787.
- Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
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