Recombinant Human Calsyntenin-2 Protein, CF

Recombinant Human Calsyntenin-2 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Calsyntenin-2

Calsyntenin-2, also called Alc-gamma (Alcadein-gamma; gene name CLSTN2) is a 120-135 kDa glycoprotein that is a member of the Alcadein family of cadherin domain‑containing type I transmembrane molecules (1, 2). Mature human Calsyntenin-2 is a 935 amino acid (aa) protein with two extracellular cadherin domains. Proteolytic cleavage by ADAM10 and gamma -secretase generates a 105-110 kDa extracellular domain termed soluble alc-gamma, and a 25 kDa, highly acidic, calcium-binding intracellular domain termed Alc gamma ICD (1-3). The soluble alc-gamma portion of human Calsyntenin-2 (aa 21-831) shares 95%, 94%, 96%, 95% and 94% aa identity with mouse, rat, canine, bovine and equine Calsyntenin-2, respectively. A potential isoform variant shows a premature truncation after Gln608. Calsyntenins are predominantly expressed in the ER/Golgi and post-synaptic plasma membranes of neurons (1). While nearly all neurons express Calsyntenin 1, Calsyntenin-2 is mainly found in subpopulations GABAergic  neurons of cortical layers 5 and 6, cerebellum (in Purkinje cells), amygdale (basolateral cells), hippocampus (pyramidal cells and hilus interneurons) and some brain stem neurons (1). Calsyntenin-2 interacts with amyloid precursor protein (APP) through its binding protein, APBA2/X11L, where it is thought to regulate APP cleavage, and likely impacts post-synaptic signaling and gene activation (2, 3). Notably, Alc gamma ICD binds APBA1/FE65 and antagonizes amyloid intracellular domain (AICD) gene activation (3). Polymorphisms of the Calsyntenin-2 gene, in combination with polymorphisms of the brain signaling regulator KIBRA, are thought to modulate episodic memory performance (4, 5).

Related Research Areas

• APP Processing and Amyloid Plaque Formation
• Calcium-binding Proteins and Related Molecules
• Synaptic Adhesion Molecules
• Synaptic Proteins