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Recombinant Human CCL20/MIP-3 alpha Protein

Catalog #: 360-MP
22 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
360-MP-025/CF

With Carrier

Catalog # Availability Size / Price Qty
360-MP-025
Recombinant Human CCL20/MIP-3 alpha Protein Bioactivity
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Product Details
Citations (22)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL20/MIP-3 alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR6. The ED50 for this effect is 0.5‑2 ng/mL.
Source
E. coli-derived human CCL20/MIP-3 alpha protein
Ala27-Met96 (Asn95Asp)
Accession #
P78556
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
8 kDa

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360-MP (with carrier)

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COA
SDS

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360-MP/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

360-MP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

360-MP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CCL20/MIP-3 alpha Protein Bioactivity View Larger

Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro-B cells transfected with human CCR6. The ED50 for this effect is 0.5‑2 ng/mL.

SDS-PAGE Recombinant Human CCL20/MIP-3 alpha Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human CCL20/MIP-3 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL20/MIP-3 alpha

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

Citations for Recombinant Human CCL20/MIP-3 alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium
    Authors: P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger
    Cell Reports, 2022-03-29;38(13):110571.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma
    Authors: KC Chang, RY Chen, YC Wang, LY Hung, LJ Medeiros, YP Chen, TY Chen, JC Yang, PM Chiang
    Scientific Reports, 2020-12-07;10(1):21342.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
    Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
    Mucosal Immunol, 2020-04-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. C-C Motif Ligand 20 (CCL20) and C-C Motif Chemokine Receptor 6 (CCR6) in Human Peripheral Blood Mononuclear Cells: Dysregulated in Ulcerative Colitis and a Potential Role for CCL20 in IL-1? Release
    Authors: HK Skovdahl, JK Damås, AVB Granlund, AE Østvik, B Doseth, T Bruland, TE Mollnes, AK Sandvik
    Int J Mol Sci, 2018-10-20;19(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CCR6 is required for ligand-induced CatSper activation in human sperm
    Authors: R Diao, T Wang, KL Fok, X Li, Y Ruan, MK Yu, Y Cheng, Y Chen, H Chen, L Mou, X Cai, Y Wang, Z Cai, X Zeng, HC Chan
    Oncotarget, 2017-09-05;8(53):91445-91458.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy
    Authors: G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang
    PLoS ONE, 2017-05-26;12(5):e0178352.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells
    Authors: Andy T Y Lau
    Proteomics Clin Appl, 2016-12-14;0(0):.
    Applications: ELISA (Standard)
  8. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016-11-28;0(0):.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells, Whole Cells
    Applications: Bioassay
  9. Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway.
    Authors: Yu, Qing, Lou, Xiang-mi, He, Yan
    PLoS ONE, 2015-03-13;10(3):e0120855.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The effects of the CCR6/CCL20 biological axis on the invasion and metastasis of hepatocellular carcinoma.
    Authors: Du, Dongshu, Liu, Yeliu, Qian, Haixin, Zhang, Bo, Tang, Xiaojun, Zhang, Ti, Liu, Weidong
    Int J Mol Sci, 2014-04-16;15(4):6441-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
    Authors: Kim S, Lewis C, Nadel JA
    J. Immunol., 2011-02-07;186(6):3392-400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
    Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
    J. Invest. Dermatol., 2010-03-11;130(6):1646-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009-10-28;1(4):4ra10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009-03-09;206(3):535-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007-07-05;82(4):946-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2006-12-29;109(9):3649-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006-11-20;203(12):2763-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006-10-01;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006-04-17;350(2):484-92.
    Species: Virus
    Sample Types: Virus, Whole Cells
    Applications: Binding Assay, Bioassay
  22. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human CCL20/MIP-3 alpha Protein
By Michele Doucet on 05/13/2016
Application: Stem/Immune cell maintenance or differentiation