Recombinant Human CD151 Fc Chimera Protein, CF

Catalog #: 1884-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1884-CD-050

Recombinant Human CD151 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD151 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha V beta 3 (Catalog # 3050-AV) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD151 Fc Chimera binds with an ED₅₀ of 0.3-1.8 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD151 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human CD151-LEL (Ala113-Arg221) Accession # P48509
N-terminus			C-terminus

Accession #

P48509

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

39 kDa

SDS-PAGE

41-55, and 90-102 kDa (non-reducible dimer), reducing conditions

Product Datasheets

1884-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1884-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Integrin aV beta 3 (Catalog # 3050-AV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD151 Fc Chimera binds with an ED₅₀ of 0.3-1.8 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CD11b/Integrin alpha M was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-55 kDa and 80 - 110 kDa, respectively.

Reconstitution Calculator

Background: CD151

Human CD151, also known as SFA-1, Tetraspanin-24, and GP27, is a palmitoylated glycoprotein in the tetraspanin superfamily. It is the first tetraspanin member to be identified as a promoter of cancer metastasis (1, 2), and it is found to participate in nearly all stages of cancer progression (3). CD-151 is normally expressed in endothelial cells, platelets, and frequently over-expressed in cancer cells (4). Mature CD-151 is a multi-pass membrane protein that contains four transmembrane domains, three cytoplasmic domains, and two extracellular loops. The region of amino acids (aa) 113-221 contains the largest extracellular loop (LEL) that involves in interacting with integrins and regulating integrin functions (5). Human CD151 LEL shares 88.9% and 87.0% aa identity with that of mouse and rat respectively. CD151 interacts with integrins such as alpha v beta 3, alpha 3 beta 1, alpha 6 beta 1, alpha 7 beta 1, and alpha 6 beta 4 to regulate their activities and thus resulting in modulation of adhesion, spreading, migration, angiogenesis, invasion and metastasis (1, 3-5). CD151 can also complex with immunoglobulin super family proteins and other tetraspanins such as CD9, CD81, and CD63 (3). Clinically, high levels of CD151 are correlated with poor prognosis in a variety of tumors (3, 4). CD151 has been implicated as a potential diagnostic marker in osteosarcoma and prostate cancer and a putative target for antibody-based immunotherapy (3). CD-151 is a key player in the formation of basement membranes in kidney and skin tissues; it is also associated with human papillomavirus (HPV) infection (6, 7).

References

Detchokul, S. et al. (2014) Br. J. Pharmacol. 171(24):5462.
Hasegawa, H. et al. (1996). Journal of Virology 70: 3258.
Sadej, R. et al. (2014) Laboratory Investigation 94:41.
Kumari, S. et al. (2015) Biomark Cancer 7:7.
Yu J. et al. (2017) Biochem. J. 474(4):589.
Scheffer, K.D. et al. (2014) Viruses 6:893.
Karamatic Crew, V. et al. (2004) Blood 104:2217.

Entrez Gene IDs

977 (Human); 12476 (Mouse); 64315 (Rat)

Alternate Names

CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151

Related Research Areas

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