Recombinant Human CD38 His-tag Biotin Protein, CF New

Catalog #: BT2404 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BT2404-050

Biotinylated Recombinant Human CD38 His-tag Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD38 His-tag Biotin Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 His-tag binds to Human CD38 Antibody (Catalog # AF2404) with an ED₅₀of 1.50-15.0 ng/mL. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD⁺) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >2200 pmol/min/μg, as measured under the described conditions.

Source

Mouse myeloma cell line, NS0-derived human CD38 protein
Val43-Ile300 with a C-terminal 6-His tag

Accession #

P28907.2

N-terminal Sequence
Analysis

Val43

Structure / Form

Biotinylated via amines

Predicted Molecular Mass

31 kDa

SDS-PAGE

40-44 kDa, under reducing conditions

Product Datasheets

BT2404

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT2404

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 His-tag binds to Human CD38 Antibody (AF2404) with an ED₅₀ of 1.50‑15.0 ng/mL.

Enzyme Activity

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Biotinylated Recombinant Human CD-38 His-tag Protein (Catalog # BT2404) is measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD38 His-tag Protein (Catalog # BT2404) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-44 kDa, under reducing conditions.

Reconstitution Calculator

Background: CD38

CD38, also known as ADP-ribosyl cyclase, converts NAD(P)+ into three separate products with calcium mobilizing ability: cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is a Type II transmembrane glycoprotein composed of an intracellular domain, a single transmembrane helix domain, and a large extracellular domain that contains the catalytic site (2). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (3, 4). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells (5, 6). CD38 is also reported to bind as a receptor to trigger signaling cascades (7, 8). Through both mechanisms, CD38 influences proliferation and trafficking (8, 9). CD38 is used as a marker for poor prognosis in chronic lymphocytic leukemia and multiple myeloma and is an attractive cancer immunotherapy drug target (8-11).

References

Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
Liu, Q. et al. (2005) Structure. 13:1331.
Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
Sun, L. et al. (1999) J. Cell Biol. 146:1161.
Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Deaglio, S. (2000) Chem. Immunol. 75:99.
Chillemi,A. et al. (2013) Mol. Med. 19:99.
Vaisitti, T. et al. (2015) Leukemia. 29:356.
Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374.
Tai, Y-T. et al. (2017) Oncotarget. 8:112166.

Long Name

Cluster of Differentiation 38

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Alternate Names

2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD_antigen: CD38; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 2.4.99.20; EC 3.2.2.6; EC:3.2.2.6; NAD(+) nucleosidase; T10