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Recombinant Human CD6 Fc Chimera Protein, CF

Catalog #: 627-CD
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
627-CD-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human CD6 protein
Human CD6
(His18-Glu398)
Accession # Q8WWJ7		 DIEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
Q8WWJ7
N-terminal Sequence
Analysis
His18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
68.2 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions

Product Datasheets

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627-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

627-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. CD6 is a 668 amino acid protein with a 24 amino acid predicted signal sequence, a 374 amino acid extracellular domain, and a 244 amino acid transmembrane region. The 626 amino acid murine homolog has also been identified. The human and murine proteins share 70% amino acid identity over their full-lengths.

The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogeneic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. The presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cells and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated.

References
  1. Gangemi, R.M. et al. (1989) J. Immunol. 143:2439.
  2. Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
  3. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  4. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  5. Whitney, G. et al. (1995) Mol. Immunol. 32:89.
  6. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  7. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  8. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  9. Pawelec, G. and H.J. Buhring (1991) Hum. Immunol. 31:165.
  10. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  11. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Aruffo, A. et al. (1997) Immunol. Today 18:498.
Entrez Gene IDs
923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)
Alternate Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6

Citations for Recombinant Human CD6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CDCP1 regulates retinal pigmented epithelial barrier integrity for the development of experimental autoimmune uveitis
    Authors: L Zhang, N Borjini, Y Lun, S Parab, G Enyindah-A, R Singh, BA Bell, VL Bonilha, AI Ivanov, DA Fox, RR Caspi, F Lin
    JCI Insight, 2022-09-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. CD318 is a ligand for CD6
    Authors: G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin
    Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6.
    Authors: Te Riet J, Helenius J, Strohmeyer N, Cambi A, Figdor C, Muller D
    J Cell Sci, 2014-02-04;127(0):1595-606.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.
    Authors: Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L
    Am J Pathol, 2012-09-07;181(5):1540-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation.
    Authors: Red-Horse K, Rivera J, Schanz A, Zhou Y, Winn V, Kapidzic M, Maltepe E, Okazaki K, Kochman R, Vo KC, Giudice L, Erlebacher A, McCune JM, Stoddart CA, Fisher SJ
    J. Clin. Invest., 2006-09-21;116(10):2643-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion.
    Authors: Fischer M, Harvima IT, Carvalho RF, Moller C, Naukkarinen A, Enblad G, Nilsson G
    J. Clin. Invest., 2006-09-07;116(10):2748-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Mitogen-activated protein kinase pathway activation by the CD6 lymphocyte surface receptor.
    Authors: Ibáñez A, Sarrias MR, Farnos M, Gimferrer I, Serra-Pages C, Vives J, Lozano F
    J. Immunol., 2006-07-15;177(2):1152-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells.
    Authors: Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG
    Blood, 2005-12-13;107(8):3212-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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