Recombinant Human CLC/CNTFR alpha Chimera Protein
Recombinant Human CLC/CNTFR alpha Chimera Protein Summary
Product Specifications
| Human CNTF R alpha (Gln23 - Pro346) Accession # P26992 | GSGSSRGGSGSGGSGGGGSKL | Human CLC (Leu28 - Phe225) Accession # Q9UBD9 |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2415-CR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2415-CR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CLC/CNTF R alpha Chimera
Cardiotrophin-like cytokine (CLC), (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3) is a 22 - 25 kDa member of the IL-6 family of cytokines (1 - 3). As such, it is expressed as a long, type I cytokine with four alpha -helices in its structure (2). Human CLC is synthesized as a 225 amino acid (aa) precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for Ciliary Neurotrophic Factor Receptor alpha (CNTF R alpha ) and Cytokine Receptor-like Factor (CLF) (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently complexed to either CLF or soluble CNTF R alpha (5, 6). Once complexed, CLC signals through a tripartite receptor complex composed of gp130, LIF R beta and CNTF R alpha (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC shares 96% aa sequence identity. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9 - 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).
- Murakami, M. et al. (2004) Growth Factors 22:75.
- Ernst, M. and B.J. Jenkins (2004) Trends Genet. 20:23.
- Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
- Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
- Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
- Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
- Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
- Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
- Forger, N.G. et al. (2003) J. Neurosci. 23:8854
- Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
- Senaldi, G. et al. (2002) J. Immunol. 168:5690.
- de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.
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