Home / CLEC9a / Recombinant Human CLEC9a Fc Chimera Protein, CF

Recombinant Human CLEC9a Fc Chimera Protein, CF

Catalog #: 6049-CL
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6049-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CLEC9a Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899. At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.
Source
Mouse myeloma cell line, NS0-derived human CLEC9a protein
Met-Asp
 Human IgG1
(Pro100-Lys330)
 IEGR Human CLEC9a
(Lys57-Val241)
Accession # Q6UXN8
N-terminus C-terminus
Accession #
Q6UXN8
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47.8 kDa (monomer)
SDS-PAGE
55-65 kDa, reducing conditions

Product Datasheets

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6049-CL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6049-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC9a

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2-4). Within the ECD, human CLEC9A shares 57% aa sequence identity with mouse and rat CLEC9A. Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (2-5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2-5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL-mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

References
  1. Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
  2. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  3. Caminschi, I. et al. (2008) Blood 112:3264.
  4. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  5. Schreibelt, G. et al. (2012) Blood 119:2284.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
  8. Sancho, D. et al. (2009) Nature 458:899.
  9. Ahrens, S. et al. (2012) Immunity 36:635.
  10. Zhang, J.-G. et al. (2012) Immunity 36:646.
  11. Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
  12. Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.
    Long Name
    C-type Lectin-like Receptor 9
    Entrez Gene IDs
    283420 (Human); 232414 (Mouse)
    Alternate Names
    CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

    Citations for Recombinant Human CLEC9a Fc Chimera Protein, CF

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    3 Citations: Showing 1 - 3
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    1. An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides
      Authors: LL Eggink, KF Roby, R Cote, J Kenneth Ho
      J Immunother Cancer, 2018-04-17;6(1):28.
      Species: Human
      Sample Types: Peptide
      Applications: Binding Assay
    2. Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity
      Authors: T Uto, T Fukaya, H Takagi, K Arimura, T Nakamura, N Kojima, B Malissen, K Sato
      Nat Commun, 2016-04-12;7(0):11273.
      Species: Human
      Sample Types: Recombinant Protein
      Applications: Enzyme Assay
    3. The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice.
      Authors: Iborra S, Izquierdo HM, Martinez-Lopez M, Blanco-Menendez N, Reis E Sousa C, Sancho D
      J. Clin. Invest., 2012-04-16;122(5):1628-43.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Flow Cytometry

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