Recombinant Human Complement Component C3a Protein, CF

Catalog #: 3677-C3
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3677-C3-025

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Citations (7)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Complement Component C3a Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). Fischer, W.H. et al. (1999) J. Immunol. 162:453. The ED₅₀ for this effect is 1.5-7.5 µg/mL.

Source

E. coli-derived human Complement Component C3a protein
Ser672-Arg748

Accession #

NP_000055

N-terminal Sequence
Analysis

Ser672

Predicted Molecular Mass

9.1 kDa

Product Datasheets

3677-C3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3677-C3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Complement Component C3a

C3a is an anaphylotoxin polypeptide comprising amino acids (aa) 672-748 of the Complement C3 precursor protein (1-4). Anaphylatoxins are proteolytically generated from the C3, C4 and C5 alpha chains by convertases formed by other complement fragments (2). They share 30-36% aa identity, and mediate inflammatory responses that vary in strength in the order C5a>C3a>C4a (2). Like C4a and C5a, the 77 aa, 9 kDa human C3a contains six conserved cysteine residues that form a knot structure and possess an overall basic charge (4, 5). It is not glycosylated (4). The C-terminal regions of C3a and C4a, but not C5a, shows antimicrobial activity (5). Human C3a shows 67-69% aa identity with mouse, rat, guinea pig, bovine, porcine and canine C3a. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative (1, 2). It binds the G-protein coupled C3a receptor (C3aR) on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells (7, 10). C3a contributes to both innate and adaptive immunity. It activates mast cells and neutrophils, triggering robust mast cell degranulation in airways during asthmatic allergen challenges (9). It enhances lipopolysaccharide-induced prostaglandin, cytokine and chemokine secretion by macrophages and other cells (1, 6-8). It assists in Th2-type inflammatory reactions and stimulates smooth muscle contraction and leukocyte chemotaxis (8, 9). Endogenous carboxypeptidase-N can remove the arginine at the C-terminus of the anaphylatoxins to create desArg forms (1). C3adesArg, also called ASP (Acylation-Stimulating Protein) is an adipocyte-derived protein that binds the C5L2 (GPR77) receptor and stimulates adipose tissue triglyceride synthesis (2, 10, 11). The anaphylactic activity of ASP is weaker than that of C3a (6, 10). C5L2 is also involved in C3a and C5a activity (11).

References

Markiewski, M. and J.D. Lambris (2007) Am. J. Pathol. 171:715.
Haas, P-J. and J. van Strijp (2007) Immunol. Res. 37:161.
DeBruijn, M.H. and G.H. Fey (1985) Proc. Natl. Acad. Sci. USA 82:708.
Hugli, T.E. (1975) J. Biol. Chem. 250:8293.
Pasupuleti, M. et al. (2007) J. Biol. Chem. 282:2520.
Fischer, W.H. et al. (1999) J. Immunol. 162:453.
Thurman, J.M. et al. (2007) J. Immunol. 178:1819.
Honczarenko, M. et al. (2005) J. Immunol. 175:3698.
Ali, H. and R.A. Panettieri (2005) Respir. Res. 6:19.
Kalant, D. et al. (2005) J. Biol. Chem. 280:23936.
Chen, N-J. et al. (2007) Nature 446:203.

Entrez Gene IDs

718 (Human); 12266 (Mouse)

Alternate Names

AHUS5;ARMD9;ASP;C3a;C3b;Complement C3;CPAMD1;HEL-S-62p; Anaphylatoxin; C3; Complement Component C3a

Related Research Areas

Citations for Recombinant Human Complement Component C3a Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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