Recombinant Human EpCAM/TROP1 Fc Alexa Fluor® 647 Protein

Catalog # Availability Size / Price Qty
AFR960-020
Detection of Human EpCAM/TROP 1 Antibody with Recombinant Human EpCAM/TROP 1 Fc Chimera Alexa Fluor® 647 Protein by Flow Cytometry.
2 Images
Product Details
FAQs
Reviews

Recombinant Human EpCAM/TROP1 Fc Alexa Fluor® 647 Protein Summary

Learn more about FluorokinesTM Fluorescent-Labeled Proteins

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by flow cytometry for its ability to bind Human EpCAM/TROP-1 Antibody conjugated fluorescent beads at 0.500-2.00 µg/mL (100 µL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human EpCAM/TROP1 protein
Human EpCAM
(Gln24-Lys265)
Accession # CAA32870.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained; Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Labeled with Alexa Fluor® 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Predicted Molecular Mass
54 kDa
SDS-PAGE
60-70 kDa, under reducing conditions.

Product Datasheets

You must select a language.

x

AFR960

AFR960

Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Flow Cytometry View Larger

Fluorescent beads conjugated to Human EpCAM/TROP 1 Antibody were stained with (A) Recombinant Human EpCAM/TROP 1 Fc Chimera Alexa Fluor® 647 (Catalog # AFR960, filled histogram) or (B) unstained (open histogram).

SDS-PAGE View Larger

2 μg/lane of Recombinant Human EpCAM/TROP‑1 Fc Chimera Alexa Fluor® 647 Protein (Catalog # AFR960) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70- kDa and 120-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with Trop‑2/EGP‑1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human EpCAM/TROP1 Fc Alexa Fluor® 647 Protein

There are currently no reviews for this product. Be the first to review Recombinant Human EpCAM/TROP1 Fc Alexa Fluor® 647 Protein and earn rewards!

Have you used Recombinant Human EpCAM/TROP1 Fc Alexa Fluor® 647 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review