Recombinant Human FCRL4/FcRH4 His-tag Protein, CF Summary
Product Specifications
Ala18-Arg385, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2426-FC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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When Recombinant Human FCRL4 (Catalog # 2426-FC) is coated onto a microplate at 0.5 µg/mL, Biotinylated Human IgA binds with an ED50 of 5-20 μg/mL.
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2 μg/lane of Recombinant Human FCRL4/FcRH4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-60 kDa and 45-52 kDa, respectively.
Reconstitution Calculator
Background: FCRL4/FcRH4
Fc receptor-like 4 (FCRL4), also known as FcRH4, IFGP2 and IRTA1, is a member of the Ig superfamily transmembrane protein, which is preferentially expressed in B cells and contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1). Mature human FCRL4 consists of a 368 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-type domains, a 21 aa transmembrane segment, and a 107 aa cytoplasmic domain with three ITIM-like motifs. FCRL4 is closely related to FC gamma RIIB (2, 3) and shows high homology to Fc receptors (4). In cellular binding assays, FCRL4 bound to IgA efficiently (5). The FCRL4 gene is localized in the human chromosome 1q21-23 region, a hotspot for translocation events (3). The B cells expressing FCRL4 participate in the autoimmune response in rheumatoid arthritis patients (6).
- Ehrhardt, G.R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:13489.
- Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. U. S. A. 98:9772.
- Hatzivassiliou, G. et al. (2001) Immunity 14:277.
- Amara, K. et al. (2007) J. Autoimmun. 81:34.
- Wilson, T.J. et al. (2012) J. Immunol. 188:4741.
- Miller, I. et al. (2002) Blood 99:2662.
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