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Recombinant Human FGF-22 Protein

Catalog #: 3867-FG
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3867-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3867-FG-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-22 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 50-300 ng/mL.
Source
E. coli-derived human FGF-22 protein
Thr23-Ser170, with an N-terminal Met and 6-His tag
Accession #
Q9HCT0
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18 kDa

Product Datasheets

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3867-FG (with carrier)

Product Datasheet
COA
SDS

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3867-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3867-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA, Sucrose and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3867-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA, Sucrose and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-22

Fibroblast growth factor-22 (FGF-22) is a 23 kDa, non-glycosylated member of the FGF-7 subfamily, from the FGF family of heparin-binding growth factors (1-3). The human FGF-22 precursor is 170 amino acids (aa) in length, and contains a 22 aa signal sequence with a 148 aa mature region (4-6). The mature region shows a centrally-placed, 120 aa beta -trefoil region (aa 43-168) that is characteristic of all FGF family members. Human FGF-22 potentially has one alternate splice form. This isoform is 129 aa in length, and shows a 31 aa substitution for the first N-terminal 72 aa of the standard, or long, form (7). There is no information related to its possible function. Mature human FGF-22 is 86% aa identical to mouse FGF-22, with the mouse molecule showing a 9 aa deletion at the N-terminus (5). FGF-22 is synthesized by at least three cell types; keratinocytes, neurons, and skeletal muscle myotubes (4, 8, 9). In neurons and myotubes, FGF-22 is presumed to function as an organizer of the presynaptic apparatus. Expressed by postsynaptic (or target) cells, FGF-22 is believed to bind to FGF R2b on the surface of innervating processes, resulting in synaptic vesicle clustering, organization, and neurite branching (8, 10). Although FGF-22 is assumed to be secreted, little can be found in expressing cell culture media. Presumably, it is bound to 34 kDa FGF-BP1, which is a molecule described as typically associated with cell membrane proteoglycans (6, 11). Thus, following secretion, FGF-22 could quickly be immobilized by FGF-BP1, only to be released at a later time, or aided by FGF-BP1 in its interaction with FGF R2b (6, 10, 11).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ornitz, D.M. and N. Itoh (2001) Genome Biol. 2:3005.1 Epub 2001 Mar 9.
  3. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  4. Beyer, T.A. et al. (2003) Exp. Cell Res. 287:228.
  5. Nakatake, Y. et al. (2001) Biochim. Biophys. Acta 1517:460.
  6. Beer, H-D. et al. (2005) Oncogene 24:5269.
  7. GenBank Accession #: EAW61176.
  8. Fox, M.A. and H. Umemori (2006) J. Neurochem. 97:1215.
  9. Umemori, H. et al. (2004) Cell 118:257.
  10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694
  11. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
Long Name
Fibroblast Growth Factor 22
Entrez Gene IDs
27006 (Human); 67112 (Mouse); 170579 (Rat)
Alternate Names
FGF22; FGF-22; fibroblast growth factor 22

Citations for Recombinant Human FGF-22 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons
    Authors: RO Costa, H Martins, LF Martins, AW Cwetsch, M Mele, JR Pedro, D Tomé, NL Jeon, L Cancedda, SR Jaffrey, RD Almeida
    Cell Rep, 2019-07-23;28(4):864-876.e6.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes
    Authors: S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme
    Sci Rep, 2019-06-11;9(1):8470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Human marrow stromal cells downsize the stem cell fraction of lung cancers by fibroblast growth factor 10.
    Authors: Kanehira M, Kikuchi T, Santoso A, Tode N, Hirano T, Ohkouchi S, Tamada T, Sugiura H, Harigae H, Ichinose M
    Mol Cell Biol, 2014-05-27;34(15):2848-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010-10-01;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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