Recombinant Human FGF-4, Animal-Free Protein

Catalog #: Qk004 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
Qk004-0050
Qk004-0500

Recombinant Human FGF-4, Animal-Free Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-4, Animal-Free Protein Summary

Product Specifications

Accession #

P08620.1

Product Datasheets

QK004

Product Datasheet

COA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Qk004

Stability & Storage:

Store lyophilized protein between -20 °C and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Scientific Data

Bioactivity

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Recombinant HumanFGF-4, Animal-Free activity is determined using the firefly luciferase reporter assay (*) in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized. EC50 = 4.3 ng/mL (306.5 pM). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

SDS-PAGE

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Recombinant human FGF-4 (Catalog # Qk004) migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Reconstitution Calculator

Background: FGF-4

FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.

Long Name

Fibroblast Growth Factor 4

Entrez Gene IDs

2249 (Human); 14175 (Mouse); 116499 (Rat)

Alternate Names

FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST