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Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

Catalog #: 11233-F9
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11233-F9-050
Recombinant Human FGF-9 (HEK293-expressed) Protein Bioactivity.
2 Images
Product Details
Citations (1)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-9 (HEK293-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1.00-5.00 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human FGF-9 protein
Ala2-Ser208 & Leu4-Ser208
Accession #
P31371.3
N-terminal Sequence
Analysis
Ala2 & Leu4
Predicted Molecular Mass
23 kDa
SDS-PAGE
23-30 kDa, under reducing conditions.

Product Datasheets

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11233-F9

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11233-F9

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured in a cell proliferation assay using Balb/3T3 Mouse Embryonic Fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1.00-5.00 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human FGF-9 (HEK293-expressed) Protein (Catalog # 11233-F9) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 23-30 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-9

Fibroblast growth factor 9 (FGF-9), also known as HBGF9 and GAF, is a member of the FGF family of secreted glycoproteins involved in mammalian skeleton morphogenesis and growth (1). The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a beta -trefoil structure (2). Mature mouse FGF-9 shares 99% and 100% aa sequence identity with human and rat FGF-9, respectively. FGF-9, along with FGF-16 and -20, form a FGF subfamily that shares 65-71% aa sequence identity, binds FGFR3(IIIb), and are efficiently secreted despite having an uncleavable, bipartite signal sequence (2-4). In addition to FGFR3(IIIb), FGF-9 binding to the IIIc splice forms of FGFR-1, -2 and -3 have been reported (1,4,6). In the mouse embryo, the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes (2, 6-11). Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton (2,10,11). Additionally, a mutation in mouse FGF-9 is responsible for Elbow knee synostosis (Eks), which causes joint fusions in the elbow and knee (6). In humans, FGF9 mutations that lower receptor binding can result multiple synostoses syndrome (SYNS) (7). Altered FGF-9 expression or function is reported in human colon, endometrial, and ovarian cancers, correlating with progression, invasiveness, and survival (12-15).

References
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  3. Miyamoto, M. et al. (1993) Mol. Cell. Biol. 13:4251.
  4. Santos-Ocampo, S. et al. (1996) J. Biol. Chem. 271:1726.
  5. Plotnikov, A.N. et al. (2001) J. Biol. Chem. 276:4322.
  6. Harada, M. et al. (2009) Nat. Genet. 41:289.
  7. Wu, X.L. et al. (2009) Am. J. Hum. Genet. 85:53.
  8. Colvin, J.S. et al. (1999) Dev. Dyn. 216:72.
  9. Lin, Y. et al. (2009) Dev. Biol. 329:44.
  10. Hung, I.H. et al. (2007) Dev. Biol. 307:300.
  11. Colvin, J.S. et al. (2001) Dev. Dyn 128:2095.
  12. Krejci, P. et al. (2009) Hum. Mutat. 30:1245.
  13. Leushacke, M. et al. (2011) PLoS ONE 6:e23381.
  14. Hendrix, N.D. et al. (2006) Cancer Res. 66:1354.
  15. Abdel-Rahman, W.M. et al. (2008) Hum. Mutat. 29:390.
Long Name
Fibroblast Growth Factor 9
Entrez Gene IDs
2254 (Human); 14180 (Mouse)
Alternate Names
FGF9; FGF-9; fibroblast growth factor 9 (glia-activating factor); Fibroblast growth factor 9; GAF; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3

Citation for Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Developmental and hormonal regulation of FBN1 and OR4M1 mRNA in bovine granulosa cells
    Authors: Maylem, ERS;Spicer, LJ;Batalha, IM;Sch�tz, LF;
    Domestic animal endocrinology
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay

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