Home / FGF basic/FGF2/bFGF / Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

Catalog #: 4114-TC
26 Citations 1 Review Datasheet / COA / SDS
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4114-TC-01M
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Recombinant Human FGF basic/FGF2/bFGF (145 aa), Tissue Culture Grade Protein SEC-MALS.
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Citations (26)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF Summary

Product Specifications

Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
The protein is used at 20 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells over 3 passages. Cells were cultured in N2 plus medium and supplemented with FGF basic every day. Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-3 ng/mL.
Source
E. coli-derived human FGF basic/FGF2/bFGF protein
Ala144-Ser288
Accession #
NP_001997
N-terminal Sequence
Analysis
Ala144
Predicted Molecular Mass
16 kDa

Product Datasheets

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4114-TC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4114-TC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 0.5-1.0 mg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant human FGF basic/FGF2/bFGF, 145 aa TC Grade (Catalog # 4114-TC) has a molecular weight (MW) of 17.2 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Cell Culture Staining for the stage-specific markers, Oct-4A, Snail, NKX2.5, and Troponin T at select time points during cardiomyocyte differentiation of iPSCs View Larger

JOY6 human iPSCs were cultured in media containing Cultrex™ Stem Cell Qualified RGF BME (3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (Catalog # 4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (5439-DK) on days 5-7 to induce cardiomyocyte differentiation. Cells were then cultured in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond. Cells were fixed and stained for stage-specific markers at select time points during the procedure. The pluripotency marker Oct-4 was detected using a Mouse Anti-Human Oct-4A Monoclonal Antibody (MAB17591). The mesoderm marker, Snail is expressed intermediately during differentiation (Day 1). It was detected using a Goat Anti-Human Snail Polyclonal Antibody (AF3639). The cardiomyocyte markers NKX2.5 and Troponin T are not present in cells during early (Day 0) and intermediate (Day1) differentiation and become more highly expressed during the later stages of differentiation (Day 7, Day 30). NKX2.5 was detected using a Goat Anti-Human NKX2.5 Polyclonal Antibody (AF2444) and Troponin T was detected using a Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (MAB1874). Snail and NKX2.5 primary antibodies were visualized with a NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (NL001). Oct-4 and Troponin T were visualized with a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (NL007).

Cell Culture Staining for the Atrial Marker, MLC2a, and the Ventricular Marker, MYH7, in iPSC-derived Cardiomyocytes View Larger

JOY6 human induced pluripotent stem cells (iPSCs) were differentiated into cardiomyocytes in media containing Cultrex™ Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (Catalog # 4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (5439-DK) on days 5-7. Following culture in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond, cells were fixed and stained for the atrial-specific marker, MLC2a, and the ventricle-specific marker, MYH7 using a Rabbit Anti-Human MYH7 Monoclonal Antibody (MAB90961).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

References
  1. Sorenson, V. et al. (2006) BioEssays 28:504.
  2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
  3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
  4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
  5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
  6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
  7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
  8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
  9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
  10. Nickel, W. (2005) Traffic 6:607.
  11. SwissProt # P09038.
  12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
  13. Bailly, K. et al. (2000) FASEB J. 14:333.
  14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Citations for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Crosstalk between PARN and EGFR-STAT3 Signaling Facilitates Self-Renewal and Proliferation of Glioblastoma Stem Cells
    Authors: Yin, J;Seo, Y;Rhim, J;Jin, X;Kim, TH;Kim, SS;Hong, JH;Gwak, HS;Yoo, H;Park, JB;Kim, JH;
    Cancer research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of Primary Cilium Length by O-GlcNAc during Neuronal Development in a Human Neuron Model
    Authors: Tian, JL;Huang, CW;Eslami, F;Mannino, MP;Mai, RL;Hart, GW;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Lysine catabolism reprograms tumour immunity through histone crotonylation
    Authors: Yuan, H;Wu, X;Wu, Q;Chatoff, A;Megill, E;Gao, J;Huang, T;Duan, T;Yang, K;Jin, C;Yuan, F;Wang, S;Zhao, L;Zinn, PO;Abdullah, KG;Zhao, Y;Snyder, NW;Rich, JN;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2
    Authors: EJ Kim, JY Kim, SO Kim, N Hong, SH Choi, MG Park, J Jang, SW Ham, S Seo, SY Lee, K Lee, HJ Jeong, SJ Kim, S Jeong, K Min, SC Kim, X Jin, SH Kim, SH Kim, H Kim
    Cell Reports, 2022-11-22;41(8):111626.
    Species: Human, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition
    Authors: L Mahoney-Sa, H Bouchaoui, I Boussaad, A Jonneaux, K Timmerman, O Berdeaux, S Ayton, R Krüger, JA Duce, D Devos, JC Devedjian
    Cell Reports, 2022-08-23;40(8):111231.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  6. Sox9 directs divergent epigenomic states in brain tumor subtypes
    Authors: D Sardar, HC Chen, A Reyes, S Varadharaj, A Jain, C Mohila, R Curry, B Lozzi, K Rajendran, A Cervantes, K Yu, A Jalali, G Rao, SC Mack, B Deneen
    Oncogene, 2022-07-15;119(29):e2202015119.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Autophagy and protein aggregation as a mechanism of dopaminergic degeneration in a primary human dopaminergic neuronal model
    Authors: E Cuevas, A Guzman, SM Burks, A Ramirez-Le, SF Ali, SZ Imam
    Toxicology reports, 2022-04-01;9(0):806-813.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers
    Authors: M Kobayashi, M Kobayashi, J Odajima, K Shioda, YS Hwang, K Sasaki, P Chatterjee, C Kramme, RE Kohman, GM Church, AR Loehr, RS Weiss, H Jüppner, JJ Gell, CC Lau, T Shioda
    Stem Cell Reports, 2022-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
    Authors: AN Cho, Y Jin, Y An, J Kim, YS Choi, JS Lee, J Kim, WY Choi, DJ Koo, W Yu, GE Chang, DY Kim, SH Jo, J Kim, SY Kim, YG Kim, JY Kim, N Choi, E Cheong, YJ Kim, HS Je, HC Kang, SW Cho
    Nature Communications, 2021-08-05;12(1):4730.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling in the developing mouse brain and human neurons
    Authors: SG Kim, S Lee, Y Kim, J Park, D Woo, D Kim, Y Li, W Shin, H Kang, C Yook, M Lee, K Kim, JD Roh, J Ryu, H Jung, SM Um, E Yang, H Kim, J Han, WD Heo, E Kim
    Nature Communications, 2021-05-11;12(1):2695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma
    Authors: L Jerby-Arno, C Neftel, ME Shore, HR Weisman, ND Mathewson, MJ McBride, B Haas, B Izar, A Volorio, G Boulay, L Cironi, AR Richman, LC Broye, JM Gurski, CC Luo, R Mylvaganam, L Nguyen, S Mei, JC Melms, C Georgescu, O Cohen, JE Buendia-Bu, A Segerstolp, M Sud, MS Cuoco, D Labes, S Gritsch, DR Zollinger, N Ortogero, JM Beechem, G Petur Niel, I Chebib, T Nguyen-Ngo, M Montemurro, GM Cote, E Choy, I Letovanec, S Cherix, N Wagle, PK Sorger, AB Haynes, JT Mullen, I Stamenkovi, MN Rivera, C Kadoch, KW Wucherpfen, O Rozenblatt, ML Suvà, N Riggi, A Regev
    Nature Medicine, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Spheroid
    Applications: Bioassay
  12. SLC6A20 transporter: a novel regulator of brain glycine homeostasis and NMDAR function
    Authors: M Bae, JD Roh, Y Kim, SS Kim, HM Han, E Yang, H Kang, S Lee, JY Kim, R Kang, H Jung, T Yoo, H Kim, D Kim, H Oh, S Han, D Kim, J Han, YC Bae, H Kim, S Ahn, AM Chan, D Lee, JW Kim, E Kim
    Embo Molecular Medicine, 2021-01-11;0(0):e12632.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells
    Authors: W Tao, A Zhang, K Zhai, Z Huang, H Huang, W Zhou, Q Huang, X Fang, BC Prager, X Wang, Q Wu, AE Sloan, MS Ahluwalia, JD Lathia, JS Yu, JN Rich, S Bao
    EMBO Mol Med, 2020-10-30;0(0):e12291.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells
    Authors: EAA Obara, D Aguilar-Mo, RD Rasmussen, A Frias, K Vitting-Se, YC Lim, KJ Elbæk, H Pedersen, L Vardouli, KE Jensen, J Skjoth-Ras, J Brennum, L Tuckova, R Strauss, C Dinant, J Bartek, P Hamerlik
    Nat Commun, 2020-09-18;11(1):4709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
    Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
    Cell Stem Cell, 2020-08-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Metabolic Reprograming via Deletion of CISH in Human iPSC-Derived NK Cells Promotes In�Vivo Persistence and Enhances Anti-tumor Activity
    Authors: H Zhu, RH Blum, D Bernareggi, EH Ask, Z Wu, HJ Hoel, Z Meng, C Wu, KL Guan, KJ Malmberg, DS Kaufman
    Cell Stem Cell, 2020-06-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Human beige adipocytes for drug discovery and cell therapy in metabolic diseases
    Authors: AM Singh, L Zhang, J Avery, A Yin, Y Du, H Wang, Z Li, H Fu, H Yin, S Dalton
    Nat Commun, 2020-06-02;11(1):2758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Mouse
    Sample Types:
    Applications: Cell Culture
  19. Quantitative modelling predicts the impact of DNA methylation on RNA polymerase II traffic
    Authors: J Cholewa-Wa, R Shah, S Webb, K Chhatbar, B Ramsahoye, O Pusch, M Yu, P Greulich, B Waclaw, AP Bird
    Proc. Natl. Acad. Sci. U.S.A., 2019-07-09;116(30):14995-15000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Activity of Selected Nucleoside Analogue ProTides against Zika Virus in Human Neural Stem Cells
    Authors: JA Bernatchez, M Coste, S Beck, GA Wells, LA Luna, AE Clark, Z Zhu, D Hecht, JN Rich, CD Sohl, BW Purse, JL Siqueira-N
    Viruses, 2019-04-20;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Mechanism of Action of Methotrexate Against Zika Virus
    Authors: S Beck, Z Zhu, MF Oliveira, DM Smith, JN Rich, JA Bernatchez, JL Siqueira-N
    Viruses, 2019-04-10;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  22. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Ursodeoxycholic acid inhibits the proliferation of colon cancer cells by regulating oxidative stress and cancer stem-like cell growth
    Authors: EK Kim, JH Cho, E Kim, YJ Kim
    PLoS ONE, 2017-07-14;12(7):e0181183.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. The FDA-approved drug sofosbuvir inhibits Zika virus infection
    Authors: Kristen M Bullard-Fe
    Antiviral Res, 2016-11-27;137(0):134-140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
    Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
    Stem Cells, 2014-03-01;32(3):770-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
    Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
    Stem Cells, 2008-08-21;26(11):2768-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What receptors does FGF basic bind?
  2. Does human FGF basic show activity on mouse cells?

    • Yes, it does. The bioassay uses NR-6 mouse fibroblast cells.  There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

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Reviews for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

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Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF
By Anonymous on 05/26/2018
Application: CellProlif