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Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF

Catalog #: 10224-FI Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10224-FI-050
Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI)  that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Fibrillin-1/FBN1 protein
Human Fibrillin-1
(Ala25-Thr660)
Accession # P35555		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P35555
N-terminal Sequence
Analysis
Ala25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
95 kDa
SDS-PAGE
97-108 kDa

Product Datasheets

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10224-FI

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10224-FI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 µg/mL.

SDS-PAGE Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 97-108 kDa and 190-220 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibrillin-1/FBN1

Fibrillins are glycoproteins forming the backbone of microfibrils in elastic and non-elastic tissues. They interact with other components of the extracellular matrix (ECM) and play essential roles in tissue development, homeostasis and repair. Fibrillin-1 is a calcium-binding protein that assembles to form the structural component of the 10-12 nm microfibrils of the ECM. The human Fibrillin-1 has multiple domains, primarily consisting of epidermal growth factor (EGF)-like and other modules (1, 2). The calcium-binding modules in some of the EGF domains provide structural stability and the characteristic rod-like shape of the protein (3-8). Mature human Fibrillin-1 shares 97% amino acid (aa) sequence identity with mature mouse Fibrillin-1. Human Fibrillin-1 is synthesized as an approximately 350-kDa precursor molecule, which is then proteolytically processed by furin into its biologically active form (9-10). Fibrillin microfibers are further engaged in a number of cell matrix interactions such as with integrins, bone morphogenetic proteins (BMPs) and the large latent complex of transforming growth factor-beta (11). Fibrillin-1 mutations are associated with a range of heritable connective disorders, including Marfan syndrome and acromelic dysplasias (11-12).

References
  1. Robertson, I. et al. (2011) Biochem. J. 433:263.
  2. Corson, G.M. et al. (1993) Genomics 17:476.
  3. Maslen, C.L. et al. (1991) Nature 352:334.
  4. Hanford, P.A. et al. (1991) Nature 353:395.
  5. Werner, J.M. et al. (2000) J. Mol. Biol. 296:1065.
  6. Downing, A.K. et al. (1996) Cell 85:597.
  7. Smallridge, R.S. et al. (2003) J. Biol. Chem. 278:12199.
  8. Reinhardt, D.P. et al. (1997) J. Biol. Chem. 272:7368.
  9. Raghunath, M. et al. (1999) J. Cell. Sci. 112:1093.
  10. Wallis, D.D. et al. (2003) J. Cell. Sci. 90:641.
  11. Jensen, S.A. et al. (2016) Biochem. J. 473:827.
  12. Sherratt, M.J. et al. (2001) Micron. 32:185.
Long Name
Fibrillin-1
Entrez Gene IDs
2200 (Human)
Alternate Names
ACMICD; Asprosin; FBN; FBN1; fibrillin 1 (Marfan syndrome); Fibrillin 1; fibrillin 15; fibrillin-1; GPHYSD2; MASS; MFS1; OCTD; SGS; SSKS; WMS; WMS2

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