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Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF

Catalog #: 1617-FZC
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1617-FZC-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated recombinant mouse Wnt-5a at 100 ng/mL and Recombinant Human Frizzled‑5 Fc Chimera dilutions, the concentration of Recombinant Human Frizzled‑5 Fc Chimera that produces 50% of the maximal binding response is found to be approximately 5‑20 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Frizzled-5 protein
Human Frizzled-5
(Ala27-Trp238)
Accession # NP_003459		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
NP_003459
N-terminal Sequence
Analysis
Ala27 and Ser28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50.1 kDa (monomer)
SDS-PAGE
60-68 kDa, reducing conditons

Product Datasheets

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1617-FZC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1617-FZC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature human Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

References
  1. Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
  2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
  3. Liu, C. et al. (2008) J. Neurosci. 28:5641.
  4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
  5. Ishikawa, T. et al. (2001) Development 128:25.
  6. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
  7. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
  8. Blumenthal, A. et al. (2006) Blood 108:965.
  9. He, X. et al. (1997) Science 275:1652.
  10. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  11. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
  12. Sahores, M. et al. (2010) Development 137:2215.
  13. Slater, P.G. et al. (2013) PLoS One 8:e78892.
Entrez Gene IDs
7855 (Human); 14367 (Mouse)
Alternate Names
C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor

Citation for Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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