Recombinant Human GDF-1 Protein Summary
Product Specifications
Asp254-Arg372
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6937-GD
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6937-GD/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 100 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GDF-1
Growth and Differentiation Factor 1 (GDF‑1) is a 12 ‑ 15 kDa secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that contains a 29 amino acid (aa) signal peptide, a 224 aa propeptide, and a 119 aa mature peptide which is released following proteolysis at a dibasic cleavage site (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7 ‑ 9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).
- Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
- Wall, N.A. et al. (2000) Dev. Biol. 227:495.
- Andersson, O. et al. (2007) Dev. Biol. 311:500.
- Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
- Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
- Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
- Andersson, O. et al. (2006) Dev. Biol. 293:370.
- Tanaka, C. et al. (2007) Genes Dev. 21:3272.
- Cheng, S.K. et al. (2003) Genes Dev. 17:31.
- Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.
Citations for Recombinant Human GDF-1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Monitoring phosphorylation and acetylation of CRISPR-mediated HiBiT-tagged endogenous proteins
Authors: Alves, J;Schwinn, M;Machleidt, T;Goueli, SA;Cali, JJ;Zegzouti, H;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
Nature Communications, 2021-12-08;12(1):7142.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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