Recombinant Human Glypican 2 Protein, CF Summary
Product Specifications
Pro18-Ser553 & Ala359-Ser553, both with a C-terminal 6-His tag & Pro18-Ala359
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2304-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Glypican 2
The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35-63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17-25% sequence similarity (1-4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).
- Filmus, J and S.B. Selleck (2001) J. Clin. Invest. 108:497.
- De Cat, B and G. David (2001) Semin. Cell Dev. Biol. 12:117.
- Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
- Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
- Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
- Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.
Citations for Recombinant Human Glypican 2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing
Authors: Balke-Want, H;Keerthi, V;Gkitsas, N;Mancini, AG;Kurgan, GL;Fowler, C;Xu, P;Liu, X;Asano, K;Patel, S;Fisher, CJ;Brown, AK;Tunuguntla, RH;Patel, S;Sotillo, E;Mackall, CL;Feldman, SA;
Molecular cancer
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer
Authors: N Li, A Quan, D Li, J Pan, H Ren, G Hoeltzel, N de Val, D Ashworth, W Ni, J Zhou, S Mackay, SM Hewitt, R Cachau, M Ho
Nature Communications, 2023-04-08;14(1):1986.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated
Authors: C Brégère, U Fisch, FS Halbeisen, C Schneider, T Dittmar, S Stricker, S Aghlmandi, R Guzman
PLoS ONE, 2023-02-17;18(2):e0279343.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Control -
Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis
Authors: S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech
Sci Rep, 2017-04-25;7(0):46543.
Species: Human
Sample Types: Antibody, Whole Cells
Applications: Bioassay, ELISA (Control)
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