Recombinant Human gp130 Protein Summary
Product Specifications
Leu24-Glu619 (Glu619Asp)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
228-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
228-GP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
- Narazaki, M. et al. (1993) Blood 82:1120.
- Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Citations for Recombinant Human gp130 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Bmi1 Regulates I?B? Degradation via Association with the SCF Complex
Authors: Y Okuyama, Y Tanaka, JJ Jiang, D Kamimura, A Nakamura, M Ota, T Ohki, D Higo, H Ogura, N Ishii, T Atsumi, M Murakami
J. Immunol., 2018-09-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression
Authors: H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura
Cancer Res, 2017-02-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling
Authors: R Adams, RJ Burnley, CR Valenzano, O Qureshi, C Doyle, S Lumb, M Del Carmen, R Griffin, D McMillan, RD Taylor, C Meier, P Mori, LM Griffin, U Wernery, J Kinne, S Rapecki, TS Baker, AD Lawson, M Wright, A Ettorre
Sci Rep, 2017-01-30;7(0):37716.
Applications: Surface Plasmon Resonance -
IL-6 trans-signaling is another pathway to upregulate Osteopontin
Cytokine, 2016-11-15;90(0):88-95.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Involvement of Spinal IL-6 Trans-Signaling in the Induction of Hyperexcitability of Deep Dorsal Horn Neurons by Spinal Tumor Necrosis Factor-Alpha
J Neurosci, 2016-09-21;36(38):9782-91.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Breakpoint Cluster Region-Mediated Inflammation Is Dependent on Casein Kinase II
J Immunol, 2016-09-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The soluble interleukin-6 receptor is a mediator of hematopoietic and skeletal actions of parathyroid hormone.
Authors: Cho S, Pirih F, Koh A, Michalski M, Eber M, Ritchie K, Sinder B, Oh S, Al-Dujaili S, Lee J, Kozloff K, Danciu T, Wronski T, McCauley L
J Biol Chem, 2013-01-07;288(10):6814-25.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
J Biol Chem, 2012-07-24;287(39):32848-59.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture), Surface Plasmon Resonance -
Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
Proc. Natl. Acad. Sci. U.S.A., 2008-09-16;105(38):14692-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
Arthritis Rheum., 2006-05-01;54(5):1662-72.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture) -
Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
Authors: Erices A, Conget P, Rojas C, Minguell JJ
Exp. Cell Res., 2002-10-15;280(1):24-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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