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Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

Catalog #: E-306
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-306-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Met1 - Arg1058 with a N-terminal GST (glutathione S-transferase) tag
Accession #
P22314.3
Predicted Molecular Mass
148 kDa

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E-306

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-306

Formulation Supplied as a 0.2 μm filtered solution in HEPES & NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions. 

References
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs
7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus
    Authors: L Kong, C Sui, T Chen, L Zhang, W Zhao, Y Zheng, B Liu, X Cheng, C Gao
    Cell Reports, 2023-03-26;42(4):112306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  2. RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination
    Authors: L Kiss, D Clift, N Renner, D Neuhaus, LC James
    Nature Communications, 2021-02-22;12(1):1220.
    Species: N/A
    Sample Types: Plasmid
    Applications: Ubiquitination
  3. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation
    Sci Rep, 2016-08-03;6(0):30955.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  4. Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions
    J Biol Chem, 2016-07-07;0(0):.
    Applications: Bioassay
  5. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6.
    Authors: Rodrigo-Brenni M, Gutierrez E, Hegde R
    Mol Cell, 2014-06-26;55(2):227-37.
    Applications: Bioassay
  6. Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation.
    Authors: Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang J
    J Immunol, 2014-04-11;192(10):4758-64.
    Species: Human
    Sample Types: Whole Cells

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