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Recombinant Human HPRG Protein, CF

Catalog #: 1869-HP
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1869-HP-025
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HPRG Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544. When 1 x 105 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human HPRG protein
Val19-Lys525, with a C-terminal 10-His tag
Accession #
P04196
N-terminal Sequence
Analysis
Val19
Predicted Molecular Mass
59 kDa
SDS-PAGE
80-85 kDa, reducing conditions

Product Datasheets

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1869-HP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1869-HP

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HPRG

Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19-254) and one His-Pro-rich region (aa 350-497) that is flanked by two Pro-rich segments (aa 276-321 and 498-525) (3, 4). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7-9). HPRG also shows antiangiogenic activity on endothelial cells (10).

References
  1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
  2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
  3. Koide, T. et al. (1986) Biochemistry 25:2220.
  4. SwissProt Accession # P04196.
  5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
  6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
  7. Donate, F. et al. (2004) Cancer Res. 64:5812.
  8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
  9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
  10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
Long Name
Histidine-rich Glycoprotein Precusor
Entrez Gene IDs
3273 (Human)
Alternate Names
DKFZp779H1622; Histidine-proline-rich glycoprotein; histidine-rich glycoprotein; HPRG; HPRGHRGP; HRG

Citations for Recombinant Human HPRG Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells
    Oncogene, 2016-08-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Insights into HER2 signaling from step-by-step optimization of anti-HER2 antibodies.
    Authors: Fu W, Wang Y, Zhang Y, Xiong L, Takeda H, Ding L, Xu Q, He L, Tan W, Bethune A, Zhou L
    MAbs, 2014-04-16;6(4):978-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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