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Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Catalog #: 305-GR
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
305-GR-050
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Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IGF‑I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) that produces 50% of the optimal binding response is found to be approximately 2‑10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932 with a C-terminal 10-His tag
Accession #
P08069
N-terminal Sequence
Analysis
Glu31 and Asp741
Predicted Molecular Mass
104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit)
SDS-PAGE
150 kDa, 120 kDa and 48 kDa, reducing conditions

Product Datasheets

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305-GR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

305-GR

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl, EDTA and Tween®-20 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha  and beta  subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta  subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References
  1. Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.
Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

Citations for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Rapid optimization and prototyping for therapeutic antibody-like molecules.
    Authors: Xu, Lihui, Kohli, Neeraj, Rennard, Rachel, Jiao, Yang, Razlog, Maja, Zhang, Kathy, Baum, Jason, Johnson, Bryan, Tang, Jian, Schoeberl, Birgit, Fitzgerald, Jonathan, Nielsen, Ulrik, Lugovskoy, Alexey A
    MAbs, 2013-02-07;5(2):237-54.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling.
    Authors: Iozzo RV, Buraschi S, Genua M
    J. Biol. Chem., 2011-08-12;286(40):34712-21.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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