Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF Summary
Product Specifications
Met1-Asn932 with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
305-GR
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl, EDTA and Tween®-20 with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Reconstitution Calculator
Background: IGF-I R/IGF1R
IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.
- Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.
Citations for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Rapid optimization and prototyping for therapeutic antibody-like molecules.
Authors: Xu, Lihui, Kohli, Neeraj, Rennard, Rachel, Jiao, Yang, Razlog, Maja, Zhang, Kathy, Baum, Jason, Johnson, Bryan, Tang, Jian, Schoeberl, Birgit, Fitzgerald, Jonathan, Nielsen, Ulrik, Lugovskoy, Alexey A
MAbs, 2013-02-07;5(2):237-54.
Species: Yeast
Sample Types: Whole Cells
Applications: Bioassay -
Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling.
Authors: Iozzo RV, Buraschi S, Genua M
J. Biol. Chem., 2011-08-12;286(40):34712-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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