Recombinant Human IL-11 R alpha Protein, CF
Recombinant Human IL-11 R alpha Protein, CF Summary
Product Specifications
Ser24-Val363, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8895-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-11 R alpha
Interleukin-11 receptor alpha (IL-11 R alpha ) is a 49 kDa type I transmembrane member of the gp130 subfamily of the hematopoietic cytokine receptor family (1-3). Mature human IL-11 R alpha consists of a 347 amino acid (aa) extracellular domain (ECD) that contains a C2 type Ig-like domain, two fibronectin type III domains, two potential glycosylation sites, and a WSxWS motif, followed by a 21 aa transmembrane region and a 31 aa cytoplasmic domain (4). Within the ECD, human IL-11 R alpha shares 84%, 82%, 90%, and 86% aa sequence identity with mouse, rat, equine, and bovine IL-11 R alpha, respectively. Alternative splicing generates an additional isoform that lacks the cytoplasmic domain (4). Upon low affinity binding to IL-11, IL-11 R alpha associates with gp130 to form a high affinity receptor complex (1, 3). gp130 also functions as a subunit in the receptors for Cardiotrophin-1, CNTF, IL-6, IL-27, IL-31, LIF, and Oncostatin M (5). IL-11 R alpha is widely expressed in adults, embryos, and embryonic stem cells (4, 6, 7). Deletion of IL-11 R alpha in female mice causes faulty decidualization, lack of decidual NK cells, and infertility (8-10). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL-11 R alpha increases the severity of experimental autoimmune encephalitis (11, 12). IL-11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL-11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 13). IL-11 R alpha enhances osteoclast differentiation and bone remodeling but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL-11 R alpha confers IL-11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha acts as an IL-11 antagonist (14-16).
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- Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
- Hilton, D.J. et al. (1994) EMBO J. 13:4765.
- Davidson, A.J. et al. (1997) Stem Cells 15:119.
- Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
- Robb, L. et al. (1998) Nat. Med. 4:303.
- Ain, R. et al. (2004) Dev. Dyn. 231:700.
- Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.
- Zhang, J. et al. (2011) J. Immunol. 187:1129.
- Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
- Curtis, D.J. et al. (1997) Blood 90:4403.
- Baumann, H. et al. (1996) J. Immunol. 157:284.
- Karow, J. et al. (1996) Biochem. J. 318:489.
Citations for Recombinant Human IL-11 R alpha Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells
Authors: M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg
International Journal of Molecular Sciences, 2022-01-27;23(3):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH
Authors: J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja
Nature Communications, 2021-01-04;12(1):66.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor
Authors: P Aryappalli, K Shabbiri, RJ Masad, RH Al-Marri, SM Haneefa, YA Mohamed, K Arafat, S Attoub, O Cabral-Mar, KB Ramadi, MJ Fernandez-, BK Al-Ramadi
Int J Mol Sci, 2019-09-05;20(18):.
Species: Insect
Sample Types: Honey
Applications: Bioassay
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Reason for Rating: 92% monomer by SEC.
