Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

Catalog #: AVI10382 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI10382-050

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 1 protein

Human IL-12 R beta 1 (Cys24-Glu540) Accession # P42701-1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P42701-1

N-terminal Sequence
Analysis

Cys24

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

85 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Product Datasheets

AVI10382

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10382

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response if found to be approximately 15-90 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL-12 R beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 180-230 kDa, respectively.

Reconstitution Calculator

Background: IL-12 R beta 1

IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).

References

Robinson R.T. (2015) Cytokine. 71:348.
Rosenzweig S.D. and Holland S.M. (2005) Immunol. Rev. 203:38.
Zou J. et al. (1997) J. Biol Chem, 272:6073.

Long Name

Interleukin 12 Receptor beta 1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Alternate Names

CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454