Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF

Catalog # Availability Size / Price Qty
11210-AP-100
Recombinant Human IL‑18 R beta /IL‑1 R7 His-tag Protein Bioactivity.
2 Images
Product Details
FAQs
Reviews

Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR) . The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.  
Source
Human embryonic kidney cell, HEK293-derived human IL-18 R beta/IL-1 R7 protein
Phe20-Arg356, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Phe20
Predicted Molecular Mass
39 kDa
SDS-PAGE
46-61 kDa, under reducing conditions.

Product Datasheets

You must select a language.

x

11210-AP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11210-AP

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (816-LR). The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL‑18 R beta /IL‑1 R7 His-tag Protein (Catalog # 11210-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 46-61 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References
  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Interleukin 18 Receptor beta
Entrez Gene IDs
8807 (Human); 16174 (Mouse)
Alternate Names
Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review