Recombinant Human IL-2 Protein, Animal-Free

Name: Recombinant Human IL-2 Protein, Animal-Free
Brand: R&D Systems
SKU: BT-002-AFL
Price: 304 USD

Catalog #: BT-002-AFL Datasheet / COA / SDS

New! Bypass reconstitution steps by using a liquid formulation of Animal-free Recombinant Human IL-2. Find out more here.

GMP Version Available: BT-002-GMP

GMP

Contains the cysteine to serine mutation found in Proleukin® (aldesleukin); Intended for preclinical researchers who may transition to GMP IL-2 for their clinical work

Lyophilized

Catalog #	Availability	Size / Price	Qty
BT-002-AFL-050
BT-002-AFL-01M

Liquid

Catalog #	Availability	Size / Price	Qty
BT-002-AFL-050/LQ
BT-002-AFL-01M/LQ

Request an Animal-Free Protein Sample. For a limited time, we are offering customers the opportunity to try a sample of any Animal-Free RUO protein at no charge. Request a sample of Animal-Free IL-2.

Recombinant Human IL-2 Protein, Animal-Free, Bioactivity.

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All IL-2 Proteins and Enzymes

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Recombinant Human IL-2 Protein, Animal-Free Summary

Learn more about Animal-Free Recombinant Proteins

Product Specifications

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.

The ED₅₀ for this effect is 0.0300-0.250 ng/mL.

The specific activity of recombinant human IL-2 is >5.00 x 10⁶ IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).

Source

E. coli-derived human IL-2 protein
Ala21-Thr153 (Cys145Ser), with and without an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Accession #

P60568.1

N-terminal Sequence
Analysis

Ala21 & Met

Predicted Molecular Mass

15.5 kDa

SDS-PAGE

13 kDa, under reducing conditions.

Product Datasheets

BT-002-AFL (lyophilized)

Product Datasheet

COA

SDS

BT-002-AFL/LQ (liquid)

Product Datasheet

COA

SDS

BT-002-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile deionized water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

BT-002-AFL/LQ

Formulation	Supplied as a 0.2 μm filtered solution in Sodium Acetate.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, ≤ -65 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity

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Animal-Free^TM Recombinant Human IL-2 (Catalog # BT-002-AFL) as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Two independent lotswere tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-Free^TM Recombinant Human IL-2.

Bioactivity

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Equivalent bioactivity of GMP (BT-002-GMP) Animal-Free (Catalog # BT-002-AFL) and RUO (BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively).

SDS-PAGE

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2 μg/lane of Animal-Free™ Recombinant Human IL‑2 Protein (Catalog # BT-002-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13 kDa.

Reconstitution Calculator

Background: IL-2

Recombinant Interleukin-2 (IL-2) is expressed in E. coli and has been engineered to contain the serine for cysteine substitution found in Proleukin^® (aldesleukin). Recombinant IL-2 is widely used in cell culture for the expansion of T cells. IL-2 is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c.

IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

IL-2 expression and concentration can have either immunostimulatory effects at high doses or immunosuppressive effects at low doses due to its preferential binding to different receptor subunits expressed by various immune cell types. This has led to the generation of recombinant IL-2 variants aimed at modifying IL-2 receptor binding for increased antitumor efficacy (14, 15). These variants are typically used in combination with immune checkpoint inhibitors instead of as a monotherapy (14). IL-2 can be genetically engineered to express in NK cells for CAR T cell therapies, and in combination with other cytokines like IL-15, can increase cell viability and proliferation (16). In addition to adoptive cell transfer and checkpoint blockade inhibitors, cancer vaccines that boost immune responses have been combined with IL-2 treatment with promising results in recent studies (15).

In cell culture, IL-2 is a frequently used cytokine for the proliferation, differentiation, and increased antibody secretion of B cells as they transform into plasma cells in vitro (17). IL-2 is also a classically used cytokine for the expansion of NK cells, early differentiated T cells and effector memory Treg cells for adoptive cell transfer cancer immunotherapy (16, 18). GMP IL-2 is a commonly used supplement for the expansion of these cell types for cellular therapies.

References

Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
Taniguchi, T. et al. (1983) Nature 302:305.
Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
Wang, X. et al. (2005) Science 310:1159.
Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
Jaleco, S. et al. (2003) J. Immunol. 171:61.
Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
Laurence, A. et al. (2007) Immunity 26:371.
Kryczek, I. et al. (2007) J. Immunol. 178:6730.
Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
Fehervari, Z.et al. (2006) Trends Immunol. 27:109.
Xue, D. et al. (2021) Antib Ther. 4:123.
Wolfarth, A.A. et al. (2022) Immune Netw. 22:e5.
Koehl, U. et al. (2015) Oncoimmunology. 5:e1115178.
Marsman, C. et al. (2022) Front. In Immunol. 13:815449.
Chamucero-Millares, J.A. et al. (2021) Cellular Immunol. 360:104257.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Manufacturing Specifications

Animal-Free Manufacturing Conditions

Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.

Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.

Bulk proteins are filtered using animal-free filters.

Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.

No impairment of biological activity.

High quality product obtained under stringent conditions.

For ex vivo research or bioproduction, additional documentation can be provided.