Home / IL-6/IL-6R alpha Complex / Recombinant Human IL-6/IL-6R alpha Protein Chimera

Recombinant Human IL-6/IL-6R alpha Protein Chimera

Catalog #: 8954-SR
9 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8954-SR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8954-SR-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-6/IL-6R alpha Protein Chimera Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2.5-12.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-6/IL-6R alpha Complex protein
Human IL-6R alpha
(Leu20-Asp358)
Accession # P08887		GGGSGGGSGGGS Human IL-6
Val30-Met212
Accession # P05231
N-terminusC-terminus
Accession #
P08887 (IL-6 R alpha) & P05231 (IL-6)
N-terminal Sequence
Analysis
Leu20 (Human IL-6R alpha)
Predicted Molecular Mass
59 kDa
SDS-PAGE
78-93 kDa, reducing conditions

Product Datasheets

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8954-SR (with carrier)

Product Datasheet
COA
SDS

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8954-SR/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8954-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8954-SR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6/IL-6R alpha Complex

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

References
  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
  6. Hirano, T. et al. (1986) Nature 324:73.
  7. Kestler, D.P. et al. (1995) Blood 86:4559.
  8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
  9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
  10. Alberti, L. et al. (2005) Cancer Res. 65:2.
  11. Murakami, M. et al. (1993) Science 260:1808.
  12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  14. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
Long Name
Interleukin 6/Interleukin 6 Receptor alpha Complex
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat)
Alternate Names
IL-6/IL-6R alpha Complex

Citations for Recombinant Human IL-6/IL-6R alpha Protein Chimera

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Role of IL-27 in Epstein-Barr virus infection revealed by IL-27RA deficiency
    Authors: Martin, E;Winter, S;Garcin, C;Tanita, K;Hoshino, A;Lenoir, C;Fournier, B;Migaud, M;Boutboul, D;Simonin, M;Fernandes, A;Bastard, P;Le Voyer, T;Roupie, AL;Ben Ahmed, Y;Leruez-Ville, M;Burgard, M;Rao, G;Ma, CS;Masson, C;Soudais, C;Picard, C;Bustamante, J;Tangye, SG;Cheikh, N;Seppänen, M;Puel, A;Daly, M;Casanova, JL;Neven, B;Fischer, A;Latour, S;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Pluripotent stem cell-derived neural progenitor cells can be used to model effects of IL-6 on human neurodevelopment
    Authors: Sarieva, K;Hildebrand, F;Kagermeier, T;Yentür, Z;Becker, K;Mayer, S;
    Disease models & mechanisms
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  3. IL-6 trans-signaling in a humanized mouse model of scleroderma
    Authors: Odell, ID;Agrawal, K;Sefik, E;Odell, AV;Caves, E;Kirkiles-Smith, NC;Horsley, V;Hinchcliff, M;Pober, JS;Kluger, Y;Flavell, RA;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states
    Authors: K Leng, IVL Rose, H Kim, W Xia, W Romero-Fer, B Rooney, M Koontz, E Li, Y Ao, S Wang, M Krawczyk, J Tcw, A Goate, Y Zhang, EM Ullian, MV Sofroniew, SPJ Fancy, MS Schrag, ES Lippmann, M Kampmann
    Nature Neuroscience, 2022-10-27;25(11):1528-1542.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha
    Authors: Z Cai, S Tian, T Klein, L Tu, LW Geenen, T Koudstaal, AE van den Bo, YB de Rijke, IKM Reiss, E Boersma, C van der Le, M Van Faasse, I Kema, DJ Duncker, KA Boomars, K Tran-Lundm, C Guignabert, D Merkus
    Scientific Reports, 2022-07-19;12(1):12326.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells
    Authors: G Carbotti, B Dozin, S Martini, C Giordano, F Scordamagl, M Croce, G Filaci, S Ferrini, M Fabbi
    Cancers, 2021-08-09;13(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH
    Authors: J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja
    Nature Communications, 2021-01-04;12(1):66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Effect of Maternal Obesity in Mice on IL-6 Levels and Placental Endothelial Cell Homeostasis
    Authors: T Kretschmer, M Schulze-Ed, EM Turnwald, R Janoschek, I Bae-Gartz, P Zentis, M Handwerk, M Wohlfarth, A Schauss, E Hucklenbru, J Dötsch, S Appel
    Nutrients, 2020-01-22;12(2):.
    Species: Human
    Sample Types: cell culture
    Applications: Bioassay
  9. Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition.
    Authors: Ohta R, Sugimura R, Niwa A, Saito M
    J Vis Exp, 2019-06-16;0(148):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IL-6/IL-6 R alpha Protein Chimera
By Anna Wang on 12/01/2017
Application: Apoptosis assay