Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Name: Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 306-IR
Rating: 5 (3 reviews)

Catalog #: 306-IR
10 Citations 3 Reviews Datasheet / COA / SDS

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306-IR-050

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Citations (10)

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Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (0.25 µg/mL, 100 µL/well) is immobilized on a goat anti‑human IgG Fc antibody (Catalog # G-102-C) coated plate, the concentration of recombinant human IL-7 that produces 50% of the optimal binding response is found to be 7.5-60 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human IL-7R alpha/CD127 protein

Human IL-7 R alpha (Glu21-Lys261) Accession # BAG62793.1	IIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

BAG62793.1

N-terminal Sequence
Analysis

Glu21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

54.5 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

306-IR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

306-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-/CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
Goodwin, R.G. et al. (1990) Cell 60:941.
Noguchi, M. et al. (1993) Science 262:1877.
Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
Jahn, T. et al. (2007) Blood 110:1840.
Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Alternate Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

Related Research Areas

Citations for Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Induction of a memory-like CD4+ T-cell phenotype by airway smooth muscle cells
Authors: Jang, JH;Zhou, M;Makita, K;Sun, R;El-Hajjar, M;Fonseca, G;Lauzon, AM;Martin, JG;
European journal of immunology
Species: Human hepegivirus
Sample Types: Whole Cells
Applications: Bioassay
Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes
Authors: P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth
European Journal of Immunology, 2021-11-01;0(0):.
Applications: ELISA Standard
Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele
Authors: H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax
Nat Commun, 2019-10-08;10(1):4575.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Standard
IL7RA haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes
Authors: J Seyfarth, C Lundtoft, K Förtsch, H Ahlert, J Rosenbauer, C Baechle, M Roden, RW Holl, E Mayatepek, S Kummer, T Meissner, M Jacobsen
Pediatr Diabetes, 2018-03-13;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis
Authors: C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen
PLoS Pathog., 2017-06-05;13(6):e1006425.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Reduced plasma levels of soluble interleukin-7 receptor during graft-versus-host disease (GVHD) in children and adults.
Authors: Poiret, Thomas, Rane, Lalit, Remberger, Mats, Omazic, Birgitta, Gustafsson-Jernberg, Asa, Vudattu, Nalini K, Ahmed, Raija, Ernberg, Ingemar, Winiarski, Jacek, Magalhaes, Isabelle, Ringden, Olle, Maeurer, Markus
BMC Immunol, 2014-06-19;15(0):25.
Species: Human
Sample Types: Plasma
Applications: ELISA (Standard)
IL-7 Restores Lymphocyte Functions in Septic Patients.
Authors: Venet F, Foray A, Villars-Mechin A, Malcus C, Poitevin-Later F, Lepape A, Monneret G
J Immunol, 2012-10-10;189(10):5073-81.
Applications: ELISA (Standard)
Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
Genes Immun, 2012-08-23;13(7):587-92.
Applications: ELISA (Standard)
Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
Authors: Faller EM, Sugden SM, McVey MJ
J. Immunol., 2010-07-26;185(5):2854-66.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009-06-15;182(12):7389-97.
Applications: ELISA (Standard)