Recombinant Human IL-9 Protein, CF

Catalog #: 11081-IL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11081-IL-010
11081-IL-050
11081-IL-100
11081-IL-01M

Recombinant Human IL‑9 Protein Bioactivity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-9 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.200-1.00 ng/mL

Source

Human embryonic kidney cell, HEK293-derived human IL-9 protein
Gln19-Ile144

Accession #

P15248.1

N-terminal Sequence
Analysis

Gln19 inferred from enzymatic pyroglutamate treatment revealing Gly20

Predicted Molecular Mass

14 kDa

SDS-PAGE

26-38 kDa, under reducing conditions.

Product Datasheets

11081-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11081-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IL-9 (Catalog # 11081-IL) induces MO7e human megakaryocytic leukemic cell proliferation. The ED₅₀ for this effect is 0.200‑1.00 ng/mL

SDS-PAGE

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2 μg/lane of Recombinant Human IL‑9 Protein (Catalog # 11081-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-38 kDa.

Reconstitution Calculator

Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4⁺ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9 (14, 15).

References

Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
Townsend, M.J. et al. (2000) Immunity 13:573.
Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
Tan, C. et al. (2010) J. Immunol. 185:6795.
Jager, A. et al. (2009) J. Immunol. 183:7169.
Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
Lu, L.-F. et al. (2006) Nature 442:997.
Stassen, M. et al. (2000) J. Immunol. 164:5549.
Yang, Y.-C. et al. (1989) Blood 74:1880.
Renauld, J.-C. et al. (1990) Cytokine 2:9.

Long Name

Interleukin 9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Alternate Names

Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40