Home / IL-9 / Recombinant Human IL-9 Protein

Recombinant Human IL-9 Protein

Catalog #: 209-ILB
10 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
209-ILB-050/CF
209-ILB-010/CF

With Carrier

Catalog # Availability Size / Price Qty
209-ILB-010
209-ILB-050
Best Seller
Recombinant Human IL-9 Protein Bioactivity
1 Image
Product Details
Citations (10)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-9 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.03-0.3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-9 protein
Gln19-Ile144
Accession #
P15248
N-terminal Sequence
Analysis
No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Gly20
Predicted Molecular Mass
14 kDa
SDS-PAGE
12-24 kDa, reducing conditions

Product Datasheets

You must select a language.

x

209-ILB (with carrier)

Product Datasheet
COA
SDS

You must select a language.

x

209-ILB/CF (carrier free)

COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

209-ILB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

209-ILB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-9 Protein Bioactivity View Larger

Recombinant Human IL-9 (Catalog # 209-ILB) induces MO7e human megakaryocytic leukemic cell proliferation. The ED50 for this effect is 0.03-0.3 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9 (14, 15).

References
  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Yang, Y.-C. et al. (1989) Blood 74:1880.
  15. Renauld, J.-C. et al. (1990) Cytokine 2:9.
Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Citations for Recombinant Human IL-9 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease
    Authors: P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell
    Cell Reports, 2022-09-27;40(13):111439.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  2. Expression and role of interleukin-9 in Vogt-Koyanagi-Harada disease
    Authors: Z Peng, S Jiang, M Wu, X Zhou, Q Wang
    Mol. Vis., 2017-07-31;23(0):538-547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Ref-1/APE1 as Transcriptional Regulator and Novel Therapeutic Target in Pediatric T-cell Leukemia
    Authors: J Ding, ML Fishel, AM Reed, E McAdams, M Czader, AA Cardoso, MR Kelley
    Mol. Cancer Ther., 2017-04-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-9 regulates allergen-specific Th1 responses in allergic contact dermatitis.
    Authors: Liu J, Harberts E, Tammaro A, Girardi N, Filler R, Fishelevich R, Temann A, Licona-Limon P, Girardi M, Flavell R, Gaspari A
    J Invest Dermatol, 2014-01-31;134(7):1903-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012-04-18;7(4):e34965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012-02-22;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Autocrine release of interleukin-9 promotes Jak3-dependent survival of ALK+ anaplastic large-cell lymphoma cells.
    Authors: Qiu L, Lai R, Lin Q, Lau E, Thomazy DM, Calame D, Ford RJ, Kwak LW, Kirken RA, Amin HM
    Blood, 2006-06-08;108(7):2407-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
    Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
    Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):797-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004-09-01;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-9 induces goblet cell hyperplasia during repair of human airway epithelia.
    Authors: Vermeer PD, Harson R, Einwalter LA, Moninger T
    Am. J. Respir. Cell Mol. Biol., 2003-03-01;28(3):286-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IL-9 Protein

There are currently no reviews for this product. Be the first to review Recombinant Human IL-9 Protein and earn rewards!

Have you used Recombinant Human IL-9 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review