Recombinant Human Integrin alpha 2b beta 3 Protein, CF
Recombinant Human Integrin alpha 2b beta 3 Protein, CF Summary
Product Specifications
| Human Integrin alpha 2b Leu32-Arg993 (R887L) Accession # P08514 | GGGSGGGS | Acidic Tail | HHHHHH |
| Human Integrin beta 3 (Gly27-Asp718) Accession # AAA52589 | HP | GGGSGGGS | Basic Tail |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7148-A2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Integrin alpha 2b beta 3
Integrin alpha 2b beta 3 (also alpha IIb beta 3 or GPIIbIIIa) is the only alpha 2b integrin and shares the beta 3 subunit only with alpha V beta 3 (1‑3). It is the non‑covalent heterodimer of type I transmembrane subunits, alpha 2b/CD41 (present as a disulfide‑linked complex of 114 kDa heavy and 22 kDa light chains) and 93 kDa beta 3/CD61 (1‑3). It is the most abundant integrin expressed by megakaryocytes and platelets, both on the surface and within alpha granules (1, 2). Deficiencies of alpha 2b beta 3 produce Glanzmann thrombasthenia, a potentially serious bleeding disorder (4). In its constitutively inactive state, alpha 2b beta 3 is flexed within the extracellular domains. Activation, either by intracellular signaling or by Mg2+ or Mn2+ binding, extends the integrin to expose the ligand binding site created by interaction of the beta 3 vWFA domain with the alpha 2b beta ‑propeller structure (1). The 962 aa human alpha 2b ECD shares 78‑83% aa sequence identity with mouse, rat, canine, equine and porcine alpha 2b while the 685 aa human beta 3 ECD shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and porcine beta 3. It is unclear whether splice variants of beta 3 that differ in the cytoplasmic domain are expressed significantly in platelets (5‑7). However, platelet expression of a beta 3 splice variant that produces a soluble 60 kDa beta 3 isoform, and an alpha 2b isoform lacking aa 948‑982, have been reported (7, 8). Active cell surface alpha 2b beta 3 adheres to fibrinogen, mediating platelet/platelet interactions that initiate a cascade of platelet activation and aggregation, extracellular matrix adhesion, formation of thrombi and clot retraction (1). It also binds matrix proteins that have an RGD motif, including fibronectin, plasminogen, prothrombin, thrombospondin and vitronectin (1, 2). Targeting of alpha 2b beta 3 by therapeutic antibodies or small molecules can inhibit formation of thrombi in patients with acute coronary syndrome, and potentially inhibits tumor angiogenesis and metastasis by blocking interaction of platelet alpha 2b beta 3 with tumor cells (1, 9).
- Kasirer-Friede, A. et al. (2007) Immunol. Rev. 218:247.
- Poncz, M. et al. (1987) J. Biol. Chem. 262:8476.
- Fitzgerald, L.A. et al. (1987) J. Biol. Chem. 262:3936.
- Franchini, M. et al. (2010) Clin. Chim. Acta 411:1.
- Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
- van Kuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
- Djaffar, I. et al. (1994) Biochem. J. 300:67.
- Bray, P.F. et al. (1990) J. Biol. Chem. 265:9587.
- Erpenbeck, L. and M.P. Schon (2010) Blood 115:3427.
Citations for Recombinant Human Integrin alpha 2b beta 3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
J. Clin. Invest., 2019-03-18;129(4):1713-1726.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis
Authors: S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber
Sci Rep, 2019-03-12;9(1):4661.
Species: Human
Sample Types: Imaging Agent
Applications: Binding Assay -
Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
PLoS ONE, 2013-11-18;8(11):e81165.
Applications: Binding Assay
FAQs
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What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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