Recombinant Human Integrin alpha V beta 3 Protein, CF
Recombinant Human Integrin alpha V beta 3 Protein, CF Summary
Product Specifications
| Human Integrin alpha V subunit (Phe31-Val992) Accession # NP_002201 |
His-Pro | GGGSGGGS | Acidic Tail |
| Human Integrin beta 3 subunit (Gly27-Asp718) Accession # AAA52589 |
His-Pro | GGGSGGGS | Basic Tail |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3050-AV
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Integrin alpha V beta 3
Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non‑covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N‑terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta‑propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine‑rich I‑EGF folds. The alpha V subunit domains termed thigh, calf‑1 and calf‑2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). The 962 aa human alpha V ECD(11) shares 92‑95% aa sequence identity with mouse, rat and bovine alpha V while the 685 aa human beta 3 ECD(12) shares 95% aa identity with equine and canine, and 89‑92% aa identity with mouse, rat and porcine beta 3. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M‑CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF‑1 to promote cancer cell proliferation and invasiveness (13, 14). Also, cell entry of several viruses is mediated by alpha V beta 3 (4, 10).
- Hynes, R. O. (2002) Cell 110:673.
- Serini, G. et al. (2006) Exp. Cell Res. 312:651.
- Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
- Xiong, J. et al. (2001) Science 294:339.
- Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
- vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
- Djaffar, I. et al. (1994) Biochem. J. 300:69.
- McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
- Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
- Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
- Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
- Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
- Somanath, P.R. et al. (2009) Angiogenesis 12:177.
- Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.
Citations for Recombinant Human Integrin alpha V beta 3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Evaluation of targeting ?V?3 in breast cancers using RGD peptide-based agents
Authors: Josefsson, A;Cortez, AG;Yu, J;Majumdar, S;Bhise, A;Hobbs, RF;Nedrow, JR;
Nuclear medicine and biology
Species: Murine polyomavirus strain A3
Sample Types: Whole Cells
Applications: Bioassay -
Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial
Authors: N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel
Nature Medicine, 2022-12-01;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma
Authors: AV Yagolovich, AA Isakova, AA Artykov, YV Vorontsova, DV Mazur, NV Antipova, MS Pavlyukov, MI Shakhparon, AM Gileva, EA Markvichev, EA Plotnikova, AA Pankratov, MP Kirpichnik, ME Gasparian, DA Dolgikh
International Journal of Molecular Sciences, 2022-10-21;23(20):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Molecular Cross-Talk between Integrins and Cadherins Leads to a Loss of Vascular Barrier Integrity during SARS-CoV-2 Infection
Authors: D Nader, SW Kerrigan
Viruses, 2022-04-25;14(5):.
Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Capture -
Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist
Authors: EG Norris, XS Pan, DC Hocking
bioRxiv : the preprint server for biology, 2022-04-11;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alphavbeta3 Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies
Authors: A Bugatti, F Filippini, M Bardelli, A Zani, P Chiodelli, S Messali, A Caruso, F Caccuri
Viruses, 2022-03-29;14(4):.
Species: Virus
Sample Types: Recombinant Protein, Virus
Applications: Bioassay, Surface Plasmon Resonance (SPR -
Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial
Authors: N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel
Nature Medicine, 2022;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
PloS Pathogens, 2021-12-15;17(12):e1010083.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
Authors: S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser
EBioMedicine, 2021-10-11;72(0):103617.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44
Authors: G Fnu, P Agrawal, GC Kundu, GF Weber
Biomolecules, 2021-05-30;11(6):.
Species: Human
Sample Types: Recombinant Protein
Applications: SPR -
Engineering bacterial outer membrane vesicles as transdermal nanoplatforms for photo-TRAIL-programmed therapy against melanoma
Authors: LH Peng, MZ Wang, Y Chu, L Zhang, J Niu, HT Shao, TJ Yuan, ZH Jiang, JQ Gao, XH Ning
Sci Adv, 2020-07-03;6(27):eaba2735.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity
Authors: A Szlachcic, M Sochacka, A Czyrek, L Opalinski, D Krowarsch, J Otlewski, M Zakrzewska
Cells, 2019-08-15;8(8):.
Species: Human
Sample Types: Recombinant Protein
Applications: Pull Down Assay -
Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
J. Clin. Invest., 2019-03-18;129(4):1713-1726.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis
Authors: S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber
Sci Rep, 2019-03-12;9(1):4661.
Species: Human
Sample Types: Imaging Agent
Applications: Binding Assay -
Integrin targeting of glyphosate and its cell adhesion modulation effects on osteoblastic MC3T3-E1 cells revealed by label-free optical biosensing
Authors: I Szekacs, E Farkas, BL Gemes, E Takacs, A Szekacs, R Horvath
Sci Rep, 2018-11-27;8(1):17401.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and ?v?3 integrin for osteoporosis therapy
Authors: Y Zur, L Rosenfeld, CA Keshelman, N Dalal, G Guterman-R, A Orenbuch, Y Einav, N Levaot, N Papo
PLoS Biol., 2018-08-24;16(8):e2002979.
Species: Yeast
Sample Types: Whole Cells
Applications: Bioassay -
Therapeutic potential of an anti-angiogenic multimodal biomimetic peptide in hepatocellular carcinoma
Authors: MA Barbhuiya, AC Mirando, BW Simons, G Lemtiri-Ch, JJ Green, AS Popel, NB Pandey, PT Tran
Oncotarget, 2017-09-21;8(60):101520-101534.
Species: Human
Sample Types: Peptide
Applications: Bioassay -
Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
Sci Rep, 2017-06-09;7(1):3185.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat.
Authors: Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T
J Biol Chem, 2015-08-28;290(42):25534-47.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Quantity and accessibility for specific targeting of receptors in tumours.
Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
Sci Rep, 2014-06-10;4(0):5232.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SAA1 polymorphisms are associated with variation in antiangiogenic and tumor-suppressive activities in nasopharyngeal carcinoma.
Authors: Lung H, Man O, Yeung M, Ko J, Cheung A, Law E, Yu Z, Shuen W, Tung E, Chan S, Bangarusamy D, Cheng Y, Yang X, Kan R, Phoon Y, Chan K, Chua D, Kwong D, Lee A, Ji M, Lung M
Oncogene, 2014-03-10;34(7):878-89.
Species: Human
Sample Types: Protein, Whole Cells
Applications: Bioassay -
Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
PLoS ONE, 2013-11-18;8(11):e81165.
Applications: Binding Assay -
Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
Gene Ther., 2009-12-01;16(12):1405-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
Reviews for Recombinant Human Integrin alpha V beta 3 Protein, CF
Average Rating: 4 (Based on 2 Reviews)
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Reason for Rating: Stable molecule that gives reproducible results even after long term (a few hours) incubation at 37C
Reason for Rating: Very low shelf life, buffer conditions are not very optimistic.
Integrin avb3 we purchased was good, but they wouldn't separate on reducing gel, the heterodimer remain strongly intact. I have used for binding assays, and in SPR it worked very well. traces of calcium might be present which inhibits integrin avb3 but has to be washed completely removing those ions.
