Recombinant Human JAM-B/VE-JAM Fc Chimera Protein, CF Summary
Product Specifications
When Recombinant Human JAM-B Fc Chimera is immobilized on goat anti-human IgG Fc Chimera antibody coated wells, J45.01 cells (100 µL/well at 106 cells/mL) adhesion is induced in a dose dependent manner after a 2 hour incubation at 37 °C. The ED50 for this effect is 10-60 ng/mL.
| Human JAM-B (Phe29 - Asn236) Accession # P57087 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1074-VJ
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: JAM-B/VE-JAM
The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (3, 5). Human JAM-B cDNA predicts a 298 amino acid residue (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site. Human JAM-B shares approximately 79% aa sequence homology with its mouse homologue. It also shares approximately 35% aa sequence homology with human JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the integrin alpha 4 beta 1. However, the JAM-B/ alpha 4 beta 1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).
The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred in the literature variously as JAM-B or JAM-3. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurand-Lions, M. et al. (2001) Blood 98:3699.
- Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
- Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
- Liang, T. et al. (2002) J. Immunol. 168:1618.
- Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
- Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.
Citations for Recombinant Human JAM-B/VE-JAM Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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High levels of endothelial ICAM-1 prohibit natalizumab mediated abrogation of CD4+ T cell arrest on the inflamed BBB under flow in vitro
Authors: Soldati, S;B�r, A;Vladymyrov, M;Glavin, D;McGrath, JL;Gosselet, F;Nishihara, H;Goelz, S;Engelhardt, B;
Journal of neuroinflammation
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcriptional and Post-Translational Regulation of Junctional Adhesion Molecule-B (JAM-B) in Leukocytes under Inflammatory Stimuli
Authors: PE Day-Walsh, B Keeble, G Pirabagar, SJ Fountain, PA Kroon
International Journal of Molecular Sciences, 2022-08-03;23(15):.
Species: Mouse
Sample Types: Control
Applications: Western Blot -
Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells.
Authors: Arcangeli M, Bardin F, Frontera V, Bidaut G, Obrados E, Adams R, Chabannon C, Aurrand-Lions M
Stem Cells, 2014-04-01;32(4):1043-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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