Recombinant Human Latent BMP-9 Protein Summary
Product Specifications
Lys23-Arg319 (pro) & Ser320-Arg429 (mature)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9624-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
9624-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human Latent BMP-9 (Catalog # 9624-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 1-6 ng/mL.
Reconstitution Calculator
Background: BMP-9
Human BMP-9, also known as growth and differentiation factor 2 (GDF2), is a member of the BMP subgroup of the TGF beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by nonparenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK1 (4, 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8, 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
- Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
- Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
- Song, J.J. et al. (1995) Endocrinology 136:4293.
- Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
- Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
- LopezCoviella,I. et al. (2000) Science 289:313.
- LopezCoviella,I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
- LopezCoviella,I. et al. (2002) J. Physiol. Paris 96:53.
- Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
- Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
- Ploemacher, R.E. et al. (1999) Leukemia 13:428.
FAQs
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How is the non covalent complex between the prodomain and mature domain of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) produced?
The prodomain fragment and mature fragment of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) are expressed from the same clone. The two different fragments are purified separately to isolate each fragment. Once each fragment has been isolated, they are mixed together to form the non covalent complex.
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