Recombinant Human LDLR Protein Summary
Product Specifications
Ala22-Arg788, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2148-LD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2148-LD/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: LDLR
The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors (1-5). Members of the family are endocytic receptors, but can also co-regulate adjacent cell-surface signaling molecules (3, 4). Many proteins in the LDL R family are cleaved by extracellular proteases to release soluble forms to the circulation, and many of these soluble forms are active (1, 6). Mature LDL R is a 120-160 kDa (depending on glycosylation) type I transmembrane glycoprotein that contains cysteine-rich complement-like repeats (class A LDL domains), calcium-binding EGF repeats, and beta ‑propeller structures (class B LDL repeats) in the extracellular domain (ECD) (1-7). A membrane-proximal Ser/Thr-rich region shows extensive O-linked glycosylation (4, 8). A cytoplasmic NPxY motif links the LDL R to clathrin pits for endocytosis, and binds to select adaptor proteins (1, 4, 8). The human LDL R ECD shares 78%, 76%, 81% and 82% aa sequence identity with mouse, rat, bovine, and porcine LDL R, respectively. LDL R is constitutively and widely expressed. Its class A LDL domains near the N-terminus bind apoB and apoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively (1, 2, 4, 9). Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol (2, 9). At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface (1, 4). Lack of LDL R expression or function causes familial hypercholesterolemia (FH) (4, 9, 10). The protease PCSK9 (proprotein convertase subtilisin/kexin type 9) can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface (10-12). Soluble forms of approximately 140 kDa and 28 kDa are reported to be released by phorbol esters or interferons, respectively (6, 7)
- Go, G.W. and A. Mani (2012) Yale J. Biol. Med. 85:19.
- Ren, G. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1059.
- Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
- Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
- Yamamoto, T. et al. (1984) Cell 39:27.
- Begg, M.J. et al. (2004) Eur. J. Biochem. 271:524.
- Fischer, D.G. et al. (1993) Science 262:250.
- Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560.
- Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
- De Castro-Oros, I. et al. (2010) Appl. Clin Genet. 3:53.
- Zhang, D.W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13045.
- Tavori, H. et al. (2013) Circulation 127:2403.
Citations for Recombinant Human LDLR Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Generation and Characterization of a Novel Small Biologic Alternative to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Antibodies, DS-9001a, Albumin Binding Domain-Fused Anticalin Protein
Authors: Y Masuda, S Yamaguchi, C Suzuki, T Aburatani, Y Nagano, R Miyauchi, E Suzuki, N Yamamura, K Nagatomo, H Ishihara, K Okuno, F Nara, G Matschiner, R Hashimoto, T Takahashi, T Nishizawa
J. Pharmacol. Exp. Ther., 2018-02-20;365(2):368-378.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
Authors: SA Felt, GN Droby, VZ Grdzelishv
J. Virol., 2017-07-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
Blood, 2014-02-27;123(9):1422-4.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII.
Authors: Kurasawa J, Shestopal S, Karnaukhova E, Struble E, Lee T, Sarafanov A
J Biol Chem, 2013-06-10;288(30):22033-41.
Species: Human
Sample Types: Protein
Applications: Binding Assay -
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013-02-19;288(15):10805-18.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor.
Authors: Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM
Biochem. J., 2009-05-01;419(3):577-84.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein.
Authors: Chien YJ, Chen WJ, Hsu WL, Chiou SS
Virology, 2008-07-21;379(1):143-51.
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay -
Effects of pH and low density lipoprotein (LDL) on PCSK9-dependent LDL receptor regulation.
Authors: Fisher TS, Lo Surdo P, Pandit S, Mattu M, Santoro JC, Wisniewski D, Cummings RT, Calzetta A, Cubbon RM, Fischer PA, Tarachandani A, De Francesco R, Wright SD, Sparrow CP, Carfi A, Sitlani A
J. Biol. Chem., 2007-05-10;282(28):20502-12.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance, TR-FRET
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